关键词: 茶油, 脱胶, 磷脂酶, 响应曲面法

Abstract: On the basis of single-factor tests, the degumming conditions of camellia oil by enzyme method were optimized through Box-Benhnken design and response surface methodology (RSM). The mathematical-regression model was established about the dependent variable (degumming rate of camellia oil) and independent variables (time, temperature and phospholipase Lecitase Ultra dosage). According to this model, the optimum degumming conditions are determined as time 4.3 h, temperature 47 ℃ and phospholipase Lecitase Ultra dosage 44 mg/kg. The response value (degumming rate) for these optimum values is 89.41% (n=3).

Key words: camellia oil, degumming, phospholipase, response surface methodology