食品科学 ›› 2008, Vol. 29 ›› Issue (9): 465-468.

• 分析检测 • 上一篇    下一篇

赭曲霉毒素A无毒体系胶体金试纸条的研制及与传统胶体金试纸条的比较

 赖卫华, 许杨, 熊勇华, 陈媛, 刘文娟   

  1. 南昌大学食品科学与技术国家重点实验室; 江西中德生物工程有限公司;
  • 出版日期:2008-09-15 发布日期:2011-12-08

Development of Colloidal Gold Strip with Mimotope Peptide for Rapid Detection of Ochratoxin A and Comparison between It and Traditional Strip

 LAI  Wei-Hua, XU  Yang, XIONG  Yong-Hua, CHEN  Yuan, LIU  Wen-Juan   

  1. 1. State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China; 2. Jiangxi Zodolabs Biotechnology Co,Ltd. Nanchang 330029,China
  • Online:2008-09-15 Published:2011-12-08

摘要: 本实验介绍了赭曲霉毒素A无毒体系胶体金试纸条的研制方法,其中包括胶体金的生产、金标抗体的制备、无毒体系试纸条的组装等步骤。所制备的赭曲霉毒素A无毒体系胶体金试纸条和传统的胶体金试纸条进行了比较,测试结果表明,赭曲霉毒素A无毒体系胶体金试纸条的检测限和检测时间与传统的胶体金试纸条相同,适合于赭曲霉毒素A现场筛查之用。

关键词: 赭曲霉毒素A, 胶体金, 试纸条, 模拟表位肽

Abstract: A method for rapid detection of ochratoxin A by colloidal gold strip with mimotope peptide was developed. Three steps were involved in the process of the preparation of the strip, including production of colloidal gold, manufacture of immuno gold and installation of the strip. Compared with the traditional strip, the strip with mimotope peptide has the same limit and time of detection. The method using mimotope peptides instead of mycotoxin is suitable to screen ochratoxin A on site.

Key words: ochratoxin A, colloidal gold, strip, mimotope peptide