大粒车前子多糖分离、纯化及单糖组成分析

食品科学 ›› 2008, Vol. 29 ›› Issue (9): 529-532.

大粒车前子多糖分离、纯化及单糖组成分析

殷军艺, 聂少平, 付志红, 谢明勇

南昌大学食品科学与技术国家重点实验室；江西中医学院附属中医院；

出版日期:2008-09-15 发布日期:2011-12-08

Isolation and Purification of Polysaccharide from Seeds of Plantago asiatica L. and Analysis of Its Monosaccharide Compositions

YIN Jun-Yi, NIE Shao-Ping, FU Zhi-Hong, XIE Ming-Yong

1. State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China; 2. Traditional Chinese Medicine Hospital,Jiangxi Chinese Medical University,Nanchang 330006,China

Online:2008-09-15 Published:2011-12-08

摘要/Abstract

摘要： 沸水法提取得到的大粒车前子粗多糖经过Sevag法脱蛋白后,运用生物大分子纯化系统(AKTA Purifier 100),采用凝胶柱层析法分离、纯化,得到一多糖组分(PLP,Plantago asiatica L.polysaccharide)。应用高效凝胶渗透色谱法(HPGPC)分析PLP纯度并测定其相对分子质量。气相色谱-质谱(GC-MS)联用分析PLP单糖组成。结果表明,PLP为均一多糖,其相对分子量约891.3kD。PLP主要由阿拉伯糖、木糖、甘露糖和半乳糖构成,其摩尔比为5.6:9.4:1.3:1。

关键词: 大粒车前子多糖, 纯化, 分子量, 单糖组成

Abstract: In this study, polysaccharides were isolated with boiling water from Plantago asiatica L., and then the protein in the isolates was removed by Sevag method. Successively, the polysaccharides were further purified using the AKTA purifier 100 system and column gel permeation. The main component, named PLP (Plantago asiatica L. polysaccharides) was obtained. Its purity and molecular weight (Mw) were determined by high performance gel permeation chromatography (HPGPC). Gas chromatography-mass spectrometry (GC-MS) was used to study the monosaccharide composition. The results showed that PLP is homogeneous. The Mw is about 891.3 kD. Arabinose, xylose, mannose and galactose are determined to be the components of PLP in the mol ratios of 5.6∶9.4∶1.3∶1.

Key words: polysaccharides from Plantago asiatica L, purification, molecular weight, monosaccharide composition

殷军艺, 聂少平, 付志红, 谢明勇. 大粒车前子多糖分离、纯化及单糖组成分析[J]. 食品科学, 2008, 29(9): 529-532.

YIN Jun-Yi, NIE Shao-Ping, FU Zhi-Hong, XIE Ming-Yong. Isolation and Purification of Polysaccharide from Seeds of Plantago asiatica L. and Analysis of Its Monosaccharide Compositions[J]. FOOD SCIENCE, 2008, 29(9): 529-532.

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