食品科学 ›› 2009, Vol. 30 ›› Issue (21): 83-86.doi: 10.7506/spkx1002-6300-200921020

• 基础研究 • 上一篇    下一篇

蛋白浓度对兔腰大肌肌球蛋白热凝胶特性的影响

白 云1,钟国良2,周光宏1 ,* ,彭增起1,徐幸莲1,秦建华2,蓝 晓2,辛 甜2   

  1. 1.南京农业大学 教育部肉品加工与质量控制重点实验室 2.南京农业大学食品科技学院
  • 收稿日期:2008-11-17 出版日期:2009-11-01 发布日期:2010-12-29
  • 通讯作者: 周光宏1 ,* E-mail:ghzhou@niau.edu.cn
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAD05A03); 国家自然科学基金项目(30771526)

Effect of Protein Concentration on Heat-induced Gel Properties of Myosin from Rabbit Psoas Major Muscles

BAI Yun1,ZHONG Guo-liang2,ZHOU Guang-hong1,*,PENG Zeng-qi1,XU Xing-lian1,QIN Jian-hua2,LAN Xiao2,XIN Tian2   

  1. 1. Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University, Nanjing
    210095, China;2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2008-11-17 Online:2009-11-01 Published:2010-12-29
  • Contact: ZHOU Guang-hong1,*, E-mail:ghzhou@niau.edu.cn

摘要:

肌球蛋白热诱导凝胶对肉糜类制品的黏结性、保水性等有很重要的影响。实验研究在pH6.5,0.6mol/LKCl 下,肌球蛋白浓度(5、10、15、20、25mg/ml)对兔腰大肌肌球蛋白热诱导凝胶超微结构、保水性、硬度、弹性的影响。结果表明:肌球蛋白浓度对肌球蛋白凝胶的凝胶特性有显著影响,随蛋白浓度的增加,凝胶网络更加细致,网孔从直径6~10μm 逐渐变小至最小值2μm,并在浓度达15mg/ml 以上时出现了蛋白聚集体。随蛋白浓度的增加,凝胶保水性从28.38% 增至86.96%,硬度从7.07g 增至36.23g,弹性由0.25 增至0.80。

关键词: 肌球蛋白, 凝胶, 超微结构, 保水性, 硬度, 弹性

Abstract:

Effects of protein concentration (25, 20, 15, 10, 5 mg/ml) diluted using phosphate buffer at pH 6.5 containing 0.6 mol/L KCl on heat-induced gel properties (ultramicrostructure, water holding capacity, hardness and elasticity) of myosin from rabbit psoas major muscles were investigated. Protein concentration revealed a significant effect on the gel properties. In gels formed at increasing myosin concentration, the gel networks became tighter, and the network diameter decreased from 6-10μm to 2 μm. When the myosin concentration reached a value above 15 mg/ml, protein aggregates appeared. Moreover, with the increasing of protein concentration, remarkable increases in water holding capacity, hardness and elasticity were observed from 28.38% to 86.96%, 7.07 to 36.23 g, and 0.25 to 0.80, respectively.

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