关键词: 水产品, 高效液相色谱, 荧光检测, 磺胺类药物

Abstract:

A method for determination of sulfonamide residues in fishery products by using high performance liquid chromatography (HPLC) equipped with fluorescence detector was established. The homogenized sample was extracted with ethyl acetate, back extracted with 2 mol/L hydrochloric acid, degreased with hexane, derived with fluorescamine, separated by reverse-phase chromatographic column, determined by a fluorescence detector and quantified with an internal standard of sulfamete. The results showed that the linear ranges of sulfadiazine, sulfamerazine, sulfamethazine, sulfamethoxazole are between 0.01 mg/kg and 0.2 mg/kg, and their linear correlation correlations are over 0.9934. Furthermore, in the ranges of 0.01 to 0.2 mg/kg, their average recoveries are between 83.0% and 91.3% with the RSDs of 3.89% to 8.80%, and their detection limits are 0.01 mg/kg.

Key words: fishery products, high performance liquid chromatography (HPLC), fluorescence detection, sulfonamide