食品科学 ›› 2010, Vol. 31 ›› Issue (24): 348-354.doi: 10.7506/spkx1002-6630-201024077

• 分析检测 • 上一篇    下一篇

番茄及其制品中全反式番茄红素及bate-胡萝卜素含量的C30-HPLC内标法定量分析

丁 靖,惠伯棣   

  1. 北京联合大学应用文理学院
  • 收稿日期:2010-09-13 修回日期:2010-11-15 出版日期:2010-12-25 发布日期:2010-12-29
  • 通讯作者: 惠伯棣 E-mail:bodi_hui@ygi.edu.cn
  • 基金资助:

    “十一五”国家科技支撑计划项目(2008BAI58B06)

Quantification of All-trans-lycopene and β-Carotene from Tomato and Its Products by Internal Standard Method on C30-HPLC

DING Jing,HUI Bo-di*   

  1. College of Applied Arts and Science, Beijing Union University, Beijing 100191, China
  • Received:2010-09-13 Revised:2010-11-15 Online:2010-12-25 Published:2010-12-29
  • Contact: Bodi HUI E-mail:bodi_hui@ygi.edu.cn

摘要:

目的:建立C30-HPLC 内标法对番茄及其制品中全反式番茄红素和β- 胡萝卜素进行同步定量检测方法。方法:色谱条件:固定相:YMC Carotenoid column C30 色谱柱 (4.6mm × 250mm,5μm);流动相A:乙腈:甲醇(3:1,V/V);流动相B:甲基叔丁基醚(MTBE);流动相A 与B 中分别添加体积分数0.05% 的三乙胺;洗脱条件:B 在0~10min 内保持30%,在10~20min 内,B 由30% 线性增至90%,20~30min 内B 维持在90%;流速:1mL/min;色谱图检测波长470nm 和450nm;进样量:20μL;柱温:室温。内标物为全反式-8'- 阿朴-8'-β- 胡萝卜素醛。结果:此方法的最小定量限为0.01μg/mL。在0.3~0.6μg 范围内,内标物对全反式番茄红素和β-胡萝卜素的相对校正因子分别为1.36 和1.22,重复性RSD 分别为小于0.50%和0.70%,加样回收率均在99.00% 以上。结论:应用本方法可对番茄及其制品中的全反式番茄红素和β- 胡萝卜素进行定量分析。

关键词: 番茄红素, β- 胡萝卜素, 8'- 阿朴-8'- β- 胡萝卜素醛, 内标

Abstract:

Objective: To establish a simultaneous determination method for all-trans-lycopene and β-carotene by internal standard on C30-HPLC. Methods: Stationary phase, YMCTM Carotenoid column C30 (4.6 mm×250 mm, SOD-5μm, Waters); mobile phase A, CH3CN-MeOH (3:1, V/V); mobile phase B, MTBE; both A and B with the addition of 0.05% triethylamine; elution, 30% B in 0-10 min, a linear increase of mobile phase B from 30% to 90% in 10-20 min and 90% mobile phase in 20-30 min; flow rate, 1 mL/min; PDA wavelength range, 300-600 nm; monitoring wavelength, 470 nm and 450 nm; injection volume, 20μL; column temperature, room temperature. Results: Minimum detection limit of this method was 0.01μg/mL. In the range of 0.3-0.6μg internal standard, relative correction factor of internal standard to all-trans-lycopene andβ-carotene were 1.36 and 1.22, respectively. The relative standard deviations of reproducibility experiments were 0.50% and 0.70%, respectively. The recovery rates of this method were higher than 99.00%. Conclusion: The established method is suitable for the determination for all-trans-lycopene and β-carotene in tomato and its products.

Key words: lycopene, β-carotene, 8'-apo-8'-β-carotenal, internal standard

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