食品科学 ›› 2011, Vol. 32 ›› Issue (9): 266-271.doi: 10.7506/spkx1002-6630-201109059

• 营养卫生 • 上一篇    下一篇

菜籽饼粕多糖WPS-1的抗肿瘤活性

朱建飞,唐春红.,吴谋成   

  1. 1. 重庆工商大学环境与生物工程学院 2. 重庆工商大学绿色食品研究所 3. 华中农业大学食品科学技术学院
  • 出版日期:2011-05-15 发布日期:2011-04-11
  • 基金资助:
    “十五”国家重大科技专项(200lBA501A20);湖北省“十一五”科技攻关项目(2006AA20lB32)

Antitumor Activity of Polysaccharide WPS-1 Derived from Rapeseed Meal

ZHU Jian-fei1,2,TANG Chun-hong1,2,WU Mou-cheng3   

  1. 1. College of Environmental and Biological Engineering, Chongqing Technology and Business University, Chongqing 400067, China;2. Natural and Health Food Research Institute, Chongqing Technology and Business University, Chongqing 400067, China;3. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Online:2011-05-15 Published:2011-04-11

摘要: 多糖WPS-1从华杂4号菜籽饼粕中分离纯化得到。采用HPLC鉴定其单糖组成,研究评价WPS-1对S180肉瘤细胞的体内外抑制作用及其对荷瘤小鼠的免疫应答作用。对瘤质量、脾质量和胸腺质量、迟发型超敏反应、巨噬细胞的吞噬作用以及脾细胞的增殖作用进行考察。结果表明WPS-1具有显著的抗肿瘤活性和免疫调节作用。

关键词: 菜籽饼粕, 免疫, 多糖, 抗肿瘤

Abstract: A polysaccharide fraction was isolated and purified from rapeseed meal Huaza No.4 and named as WPS-1. The monosaccharide composition of WPS-1 was identified by HPLC, and its inhibitory effect on the in vitro and in vivo proliferation of Sarcoma-180 (S180) tumor cells was evaluated. Meanwhile, the effect on the immune response in S180-bearing mice was tested based on tumor weight, spleen weight, thymus weight, delayed-type hypersensitivity reaction to dinitrofluorobenzene (DNFB), the phagocytic capacity of peritoneal macrophages and splenocytes proliferation. Our findings indicates that WPS-1 have significant antitumor and immunomodulating functions.

Key words: polysaccharide, rapeseed meal, antitumor, immunological

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