具有广谱抑菌活性乳酸菌的筛选及抑菌物质分析

食品科学 ›› 2012, Vol. 33 ›› Issue (1): 162-165.

具有广谱抑菌活性乳酸菌的筛选及抑菌物质分析

马妙莲1，赵静1，陈晓琳1，张付海2，朱敏2，张明1,*

1.安徽农业大学生命科学学院 2.安徽省环境监测中心站

收稿日期:2011-01-30 修回日期:2012-01-09 出版日期:2012-01-15 发布日期:2012-01-12
通讯作者: 张明 E-mail:zhangming@ahau.edu.cn
基金资助:
国家863计划项目;安徽省高校“十一五”优秀人才计划

Isolation of Lactic Acid Bacteria with Broad-Spectrum Antimicrobial Activity and Analysis of Antimicrobial Substances

,MIng ZHANG

Received:2011-01-30 Revised:2012-01-09 Online:2012-01-15 Published:2012-01-12
Contact: MIng ZHANG E-mail:zhangming@ahau.edu.cn

摘要/Abstract

摘要： 采用双层平板法从食用小米中分离出一株对实验所测的革兰氏阳性菌、阴性菌和霉菌有明显抑制作用的菌株，通过生理生化特性和16S rRNA基因序列同源性分析，确定该菌株为戊糖乳杆菌(Lactobacillus pentosus)。经气相色谱-质谱联用仪和高效液相色谱初步分析该菌株发酵上清液中含有乳酸、乙酸、琥珀酸、柠檬酸、棕榈酸、油酸、硬脂酸和亚油酸等多种抑菌成分。

关键词: 乳酸菌, 抑菌, 有机酸

Abstract: A strain with strong inhibitory activity against Gram-positive bacteria, Gram-negative bacteria and mold, named as LPEM818, was isolated from Millet by the overlay plate method. The strain was identified as Lactobacillus pentosus by physiological and biochemical characterization and 16S rRNA sequence homology analysis. In the cell-free fermentation supernatant, some antimircrobial substances including lactic acid, acetic acid, succinic acid, citric acid, palmitic acid, oleic acid, stearic acid and linoleic acid were found by GC-MS and HPLC analyses.

Key words: lactic acid bacteria, antibacteria, organic acid

中图分类号:

Q 935

马妙莲1，赵静1，陈晓琳1，张付海2，朱敏2，张明1,*. 具有广谱抑菌活性乳酸菌的筛选及抑菌物质分析[J]. 食品科学, 2012, 33(1): 162-165.

MIng ZHANG. Isolation of Lactic Acid Bacteria with Broad-Spectrum Antimicrobial Activity and Analysis of Antimicrobial Substances[J]. FOOD SCIENCE, 2012, 33(1): 162-165.

参考文献

参考文献
[1] 刘付香, 李玲, 梁炫强. 生物防治黄曲霉毒素污染研究进展[J]. 中国生物防治, 2010: 96-101.
[2] DE MARTINIS E, FRANCO B. Inhibition of foodborne pathogens by bacteriocin-producing leuconostoc sp and lactobacillus sake isolated from'linguica frescal'[J]. Revista de Microbiologia, 1997, 28(4): 284-287.
[3] LUDERS T, BIRKEMO G, FIMLAND G, et al. Strong synergy between a eukaryotic antimicrobial peptide and bacteriocins from lactic acid bacteria[J]. Applied and environmental microbiology, 2003, 69(3): 1797.
[4] HE Z, KISLA D, ZHANG L, et al. Isolation and identification of a paenibacillus polymyxa strain that coproduces a novel lantibiotic and polymyxin[J]. Applied and environmental microbiology, 2007, 73(1): 168.
[5] DE CARVALHO K, BAMBIRRA F, KRUGER M, et al. Antimicrobial compounds produced by lactobacillus sakei subsp. Sakei 2a, a bacteriocinogenic strain isolated from a brazilian meat product[J]. Journal of Industrial Microbiology and Biotechnology, 2010, 37(4): 381-390.
[6] LAVERMICOCCA P, VALERIO F, EVIDENTE A, et al. Purification and characterization of novel antifungal compounds from the sourdough lactobacillus plantarum strain 21B[J]. Applied and environmental microbiology, 2000, 66(9): 4084.
[7] 曹冬梅, 张洪英, 何成华, 等. 弯曲乳酸杆菌 HB02 抑制黄曲霉生长及产毒[J]. 南京农业大学学报, 2008, 31(003): 125-129.
[8] 唐雨蕊, 倪学勤, 曾东. 乳酸杆菌对黄曲霉生长抑制的研究[J]. 中国饲料, 2008(005): 42-45.
[9] SMAOUI S, ELLEUCH L, BEJAR W, et al. Inhibition of fungi and gram-negative bacteria by bacteriocin bacTN635 produced by lactobacillus plantarum sp. TN635[J]. Applied biochemistry and biotechnology, 2010: 1-15.
[10] 凌代文, 东秀珠. 乳酸细菌分类鉴定及实验方法[M].北京: 中国轻工业出版社, 1999.
[11] R.E.布坎南, N.E.吉本斯, 伯杰细菌鉴定手册[M].北京: 科学出版社, 1984.
[12] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M].北京: 科学出版社, 2001.
[13] 张龙翔, 张庭芳, 李令媛, 生化实验方法和技术[M]. 北京: 高等教育出版社, 1997.
[14] MAGNUSSON J, SCHNURER J. Lactobacillus coryniformis subsp. Coryniformis strain si3 produces a broad-spectrum proteinaceous antifungal compound[J]. Applied and environmental microbiology, 2001, 67(1): 1.
[15] 盛海圆, 郭艳萍, 常艳, 等. 传统泡菜中乳酸菌多样性的分析[J]. 中国微生态学杂志, 2010, 7.
[16] 朱晓兰, 高芸, 苏庆德. 毛细管气相色谱法测定番茄中的非挥发性有机酸, 高级脂肪酸和低分子量糖类[J]. 食品科学, 2004, 25(009): 152-157.
[17] 贾春晓, 熊卫东, 毛多斌, 等. 拐枣果梗中有机酸成分的GC-MS分析[J]. 中国食品学报, 2005, 5(001): 72-74.
[18] 张秀红, 刘婷婷. 具广谱抑菌活性乳酸菌的筛选[J]. 乳业科学与技术, 2010, 33(002): 56-59.
[19] SCHILLINGER U, LUCKE F. Antibacterial activity of lactobacillus sake isolated from meat[J]. Applied and environmental microbiology, 1989, 55(8): 1901-1906.
[20] Simpson, C. & Sofos, J. Antimicrobial Ingredients[J]. Ingredients in Meat Products, 301-377 (2009).
[21] Agoramoorthy, G., Chandrasekaran, M., Venkatesalu, V. et al. Antibacterial and antifungal activities of fatty acid methyl esters of the blind-your-eye mangrove from India[J]. Brazilian Journal of Microbiology 38, 739-742 (2007).
[22] LIU S, RUAN W, LI J, et al. Biological control of phytopathogenic fungi by fatty acids[J]. Mycopathologia, 2008, 166(2): 93-102.
[23] WALTERS D, RAYNOR L, MITCHELL A, et al. Antifungal activities of four fatty acids against plant pathogenic fungi[J]. Mycopathologia, 2004, 157(1): 87-90.