食品科学 ›› 2012, Vol. 33 ›› Issue (1): 209-214.

• 生物工程 • 上一篇    下一篇

诱导物对出芽短梗霉合成木聚糖酶和阿魏酰低聚糖的调控影响

张雨青1,余晓红1,2,*,顾振新2,屠 康2   

  1. 1.盐城工学院化学与生物工程学院 2.南京农业大学食品科技学院
  • 收稿日期:2011-09-25 修回日期:2012-01-08 出版日期:2012-01-15 发布日期:2012-01-12
  • 通讯作者: 余晓红 E-mail:yxh1127@163.com
  • 基金资助:

    江苏省社会发展科技支撑基金资助项目;江苏省高校自然科学研究计划基金资助项目;江苏省农业科技支撑基金资助项目;江苏省新型环保重点实验室开放课题基金资助项目

Effect of Inducers on Xylanase Activity and Biosynthesis of Ferulic Oligosaccharides in Aureobasidium pullulans

Xiao Hong-Yu   

  • Received:2011-09-25 Revised:2012-01-08 Online:2012-01-15 Published:2012-01-12
  • Contact: Xiao Hong-Yu E-mail:yxh1127@163.com

摘要: 以麦麸为原料诱变选育的不产黑色素出芽短梗霉为发酵菌株,利用其发酵过程中合成的木聚糖酶水解麦麸纤维,制备阿魏酰低聚糖(FOs)。研究碳源、氮源、金属离子和表面活性剂等诱导物对出芽短梗霉木聚糖酶活力和FOs合成的影响,以探明各物质对出芽短梗霉木聚糖酶活力和FOs合成的影响;利用正交试验设计方法研究各物质添加量对出芽短梗霉产酶和FOs的影响,确定芽短梗霉发酵制备FOs的最佳培养基。结果表明:50g/L麦麸处理液中添加15g/L葡萄糖、1g/L蛋白胨和1g/L玉米浆,FOs产量和木聚糖酶活力最高分别达到627nmol/L和52.66IU/mL。

关键词: 麦麸, 阿魏酰低聚糖, 诱导物, 合成调控, 出芽短梗霉

Abstract: Feruloyl oligosaccharides (FOs) could be produced as a result of the hydrolysis of wheat bran fiber by xylanase synthesized during fermentation by a mutant strain of Aureobasidium pullulans without the secretion of melanin bred using wheat bran. The effects of inducers such as carbon source, nitrogen source, metal ion and surfactant on the activity of xylanase and the formation of FOs were investigated. The medium composition for FOs production by the strain was optimized using the orthogonal array design method. The highest Fos production and xylanase activity, 627 nmol/L and 52.66 IU/mL, respectively, were achieved by using a medium comprised of 50 g/L wheat bran, 15 g/L glucose, 1 g/L peptone and 1 g/L corn syrup.

Key words: wheat bran, ferulic oligosaccharides, inducer, biosynthesis and control, Aureobasidium pullulans

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