葡萄籽和皮萃取物对小鼠乳腺癌细胞迁移的影响

食品科学 ›› 2012, Vol. 33 ›› Issue (1): 239-243.

葡萄籽和皮萃取物对小鼠乳腺癌细胞迁移的影响

梁志强1，陈秋宇2，孙向军2,*

1.山西农业大学食品科学与工程学院 2.上海交通大学农业与生物学院

收稿日期:2011-02-28 修回日期:2012-01-04 出版日期:2012-01-15 发布日期:2012-01-12
通讯作者: 孙向军 E-mail:xjsun@sjtu.edu.cn
基金资助:
上海市科委技术标准专项

Effects of Grape Seed and Grape Skin Extracts on the Migration of Mouse Mammary Carcinoma Cells

Received:2011-02-28 Revised:2012-01-04 Online:2012-01-15 Published:2012-01-12

摘要/Abstract

摘要： 目的：研究葡萄籽和皮萃取物对乳腺癌细胞迁移的抑制作用，以及对细胞活力的影响。方法：以小鼠乳腺癌细胞4T1为研究对象，四甲基偶氮唑盐(MTT)实验检测细胞增殖活性；细胞迁移实验检测细胞迁移能力；结果：质量浓度为5μg/mL的葡萄籽和皮萃取物对乳腺癌细胞的迁移具有明显的抑制作用，该抑制作用与药物质量浓度呈现剂量依赖关系；萃取物对癌细胞的活力有明显的抑制作用，葡萄籽和皮萃取物的半数有效质量浓度(IC50)分别为65.427μg/mL和72.408μg/mL。

关键词: 葡萄籽、皮萃取物, 鼠乳腺癌细胞, 细胞活力, 细胞迁移

Abstract: Objective: To explore the effects of grape seed and grape skin extracts on the migration, viability and proliferation of breast cancer cells. Methods: The viability of mouse mammary carcinoma 4T1 cells was determined by MTT assay and the migration ability by transwell chemotaxis assay. Results: Both grape seed and grape skin extracts significantly reduced the migration of the 4T1 cells in a dose-dependent manner, and exhibited an obvious inhibitory effect on cell migration at a concentration as low as 5μg/mL. The extracts also obviously inhibited the viability and proliferation of the 4T1 cells with IC50 (half maximal inhibitory concentration) of 65.427μg/mL and 72.408μg/mL, respectively.

Key words: grape seed and grape skin extracts, mouse mammary carcinoma cells, viability, migration

中图分类号:

Q279

梁志强1，陈秋宇2，孙向军2,*. 葡萄籽和皮萃取物对小鼠乳腺癌细胞迁移的影响[J]. 食品科学, 2012, 33(1): 239-243.

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