梅花鹿胎盘发酵制备多肽的工艺条件优化

食品科学 ›› 2012, Vol. 33 ›› Issue (17): 229-233.

梅花鹿胎盘发酵制备多肽的工艺条件优化

秦思文，岳喜庆*

沈阳农业大学食品学院

收稿日期:2011-08-05 修回日期:2012-08-08 出版日期:2012-09-15 发布日期:2012-11-09
通讯作者: 秦思文 E-mail:qinsiwen1987@126.com

Optimization of Fermentation Conditions for Polypetide Production from Sika Deer Placenta

Si-Wen Qin

Received:2011-08-05 Revised:2012-08-08 Online:2012-09-15 Published:2012-11-09
Contact: Si-Wen Qin E-mail:qinsiwen1987@126.com

摘要/Abstract

摘要： 以梅花鹿胎盘为原料，利用枯草芽孢杆菌发酵制备胎盘多肽。以多肽得率和水解度为指标通过单因素试验结合响应面分析法优化发酵条件，确定梅花鹿胎盘发酵制备多肽的最佳发酵条件为：接种量2%、起始pH7.5、发酵温度31℃、发酵时间53h、摇瓶转速130r/min。经验证，在该最佳工艺条件下多肽得率可达55.43%。

关键词: 梅花鹿胎盘, 枯草芽孢杆菌, 发酵, 多肽, 响应面分析法

Abstract: Sika deer placenta was fermented with Bacillus subtilis to produce polypeptides. The fermentation conditions were optimized by one-factor-at-a-time method and response surface methodology based on polypeptide yield and degree of hydrolysis. The optimal fermentation conditions were determined as 2% of inoculum size, initial medium pH 7.5, 31 ℃ of fermentation temperature, 53 h of fermentation time, and 130 r/min of shake flask speed. Under these conditions, the actual polypeptide yield was 55.43%.

Key words: sika deer placenta, Bacillus subtilis, fermentation, polypeptide, response surface methodology

中图分类号:

TS209

秦思文，岳喜庆*. 梅花鹿胎盘发酵制备多肽的工艺条件优化[J]. 食品科学, 2012, 33(17): 229-233.

Si-Wen Qin. Optimization of Fermentation Conditions for Polypetide Production from Sika Deer Placenta[J]. FOOD SCIENCE, 2012, 33(17): 229-233.

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