神农香菊全草精油的化学成分及抑菌机理研究

食品科学 ›› 2012, Vol. 33 ›› Issue (17): 35-39.

神农香菊全草精油的化学成分及抑菌机理研究

吴慧娟1，黄杨名2，陈科廷1，闫云君1，徐莉1，刘云1,3,*

1.华中科技大学生命科学与技术学院 2.武汉神农天香生物科技有限公司 3.北京化工大学生命科学与技术学院

收稿日期:2011-07-18 修回日期:2012-07-30 出版日期:2012-09-15 发布日期:2012-11-09
通讯作者: 刘云 E-mail:liuyunprivate@sina.com
基金资助:
国家自然科学基金

Chemical Composition and Antimicrobial Mechanism of Essential Oil from Dendranthema indicum var. aromaticum

Received:2011-07-18 Revised:2012-07-30 Online:2012-09-15 Published:2012-11-09
Contact: Liu Yun E-mail:liuyunprivate@sina.com

摘要/Abstract

摘要： 采用气相色谱-质谱联用法分析神农香菊全草精油的化学成分，并采用滤纸片琼脂扩散法和常量稀释法对其抑菌活性进行探讨；同时，运用透射电子显微镜及SDS-PAGE对其抑菌机理进行研究。结果表明：神农香菊精油含有149种组分，鉴定出63种成分，主要成分为萜烯类(13.99%)、醇类(25.82%)和醛酮类(3.69%)；神农香菊精油对4种供试细菌均有抑菌作用，在实验质量浓度下，精油抑抑菌圈直径8.15～10.90mm，对革兰氏阳性菌的抑菌效果明显优于革兰氏阴性菌；透射电子显微镜和SDS-PAGE分析表明，神农香菊全草精油抑菌机制可能在于破坏细菌细胞壁，导致细菌细胞变形和菌体蛋白质减少，直至菌体分解为碎片。

关键词: 神农香菊, 精油, 气相色谱-质谱法, 抑菌机理

Abstract: The chemical composition of essential oil isolated from the whole plant of Dendranthema indicum var. aromaticum was investigated by gas chromatography-mass spectrometry (GC-MS), and its antimicrobial activity was evaluated against 4 bacterial strains by filter paper agar diffusion and constant dilution methods. Meanwhile, the antimicrobial mechanism was explored by transmission electron microscopy (TEM) and sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that 149 components were detected in the essential oil and 63 compounds were identified. The major compounds included terpenes (13.99%), alcohols (25.82%), and aldehydes and ketones (3.69%). The essential oil had obvious inhibitory effect on all four tested species of bacteria with an inhibitory ring diameter of 8.15—10.90 mm. Its inhibitory activity against Gram-positive bacteria was much higher than against Gram-negative bacteria. TEM and SDS-PAGE analyses revealed that the antimicrobial mechanism may be achieved by damaging bacterial cell walls, causing deformed bacterial cells and reduced protein content until the cells are broken into pieces.

Key words: Dendranthema indicum var. aromaticum, essential oil, gas chromatography-mass spectrometry(GC-MS) , antibacterial mechanism

中图分类号:

TS201.2

吴慧娟1，黄杨名2，陈科廷1，闫云君1，徐莉1，刘云1,3,*. 神农香菊全草精油的化学成分及抑菌机理研究[J]. 食品科学, 2012, 33(17): 35-39.

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