乙酸均相体系中脉冲电场对壳聚糖降解的影响

食品科学 ›› 2012, Vol. 33 ›› Issue (17): 6-9.

乙酸均相体系中脉冲电场对壳聚糖降解的影响

罗文波，唐超，曾新安*

华南理工大学轻工与食品学院

收稿日期:2011-09-06 修回日期:2012-07-22 出版日期:2012-09-15 发布日期:2012-11-09
通讯作者: 曾新安 E-mail:xazeng@scut.edu.cn
基金资助:
国家自然科学基金项目;广州市科技攻关项目

Chitosan Degradation by Pulsed Electric Field in Acetic Acid Homogeneous Phase

Received:2011-09-06 Revised:2012-07-22 Online:2012-09-15 Published:2012-11-09

摘要/Abstract

摘要： 以脉冲电场为处理手段，研究电场强度、处理时间、壳聚糖质量浓度和电导率等因素对壳聚糖大分子降解的影响。结果表明：壳聚糖降解产物的相对分子质量随着电场强度的增大而降低，且在高场强下降解显著，电场强度为33.3kV/cm时，降解率达54.2%；降解速率随处理时间的延长逐渐变缓，前20min和后70min的降解率分别为18.5%和8.5%；而壳聚糖质量浓度和电导率的升高则不利于壳聚糖的降解。傅里叶变换红外光谱分析(FT-IR)分析表明，降解前后壳聚糖的侧链结构未发生改变；原料及经相同方法处理过的降解产物在2θ为10.4°和20.2°处均存在X射线衍射峰，表明产物的晶体结构没有发生明显变化。

关键词: 壳聚糖, 脉冲电场, 相对分子质量, 降解

Abstract: The effects of electric field strength, reaction time, chitosan concentration and solution conductivity on the degradation rate of chitosan were studied by pulsed electric field (PEF) treatment. The results showed that the molecular weight of chitosan revealed a decrease trend with increasing electric field strength, and a significant degradation was observed at high electric field strength. The degradation rate increased up to 54.2% after PEF treatment at 33.3 kV/cm; the degradation rate revealed a reduction with prolonged reaction time by 18.5% and 8.5% over the first 20 min and the last 70 min, respectively. Increasing solution concentration or conductivity was unfavorable for the degradation of chitosan. Infrared spectral analysis indicated that the structure of chitosan was not changed during degradation. The X-ray diffraction patterns of original chitosan and degraded chitosan exhibited a similar structure with characteristic peaks at 2θ of 10.4° and 20.2°, suggesting that degraded chitosan maintains its crystal structure.

Key words: chitosan, PEF, relative molecular weight, degradation

中图分类号:

TS24

罗文波，唐超，曾新安*. 乙酸均相体系中脉冲电场对壳聚糖降解的影响[J]. 食品科学, 2012, 33(17): 6-9.

参考文献

[1] 蒋挺大. 壳聚糖[J].化学工业出版社, 2007,: 1- 8
[2]韩永萍, 林强.低聚壳聚糖制备及其生理活性进展[J].化学工业与工程,2007,(3):272-276
[3]陈巍, 罗志刚, 李忠彦等.物理方法在壳聚糖降解中的应用[J].酿酒,2006,(2):57-60
[4]LIU Hui, BAO Jianguo, DU Yumin, et al.Effect of ultrasonic treatment on the biochemphysical properties of chitosan[J].Carbohydr. Polym,2006,(64):553-559
[5]QIN Caiqin, LI Huirong, XIAO Qi, et al.Water-solubility of chitosan and its antimicrobial activity[J].Carbohydr. Polym,2006,(63):367-374
[6]QIN Caiqin, ZHOU Bo, ZENG Lintao, et al.The physicochemical properties and antitumor activity of cellulase-treated chitosan[J].Food Chem,2004,(84):107-115
[7]CHIEN P J, SHEU F, LIN H R.Coating citrus (Murcott tangor) fruit with low molecular weight chitosan increases postharvest quality and shelf life[J].,2007,100(3):1160-1164
[8]段杉, 彭喜春, 彭志英.低聚壳聚糖的制备及应用[J].中国食品添加剂,2002,(5):66-71
[9]KHOR E, LIM L Y.Implantable applications of chitin and chitosan[J].,2003,24(13):2339-2349
[10]BODNAR M, HARTMANN J F, BORBELY J.Preparation and characterization of chitosan-based nanoparticles[J].Biomacromolecules,2005,6(5):2521-2527
[11]MACQUARRIE D J, HARDY J J E.Applications of functionalized chitosan in catalysis[J].Ind. Eng. Chem. Res,2005,44(23):8499-8520
[12]LIU Tseying, Chen Sanyuan, LIN Yiling, et al.Synthesis and characterization of amphiphatic carboxymethyl- hexanoyl chitosan hydrogel: water-retention ability and drug encapsulation[J].Lanqmuir,2006,22(23):9740-9745
[13]GEORGE M, ABRAHAM T E.Polyionic hydrocolloids for the intestinal delivery of protein drugs: Alginate and chitosan: A review[J].J. of Controlled Release,2006,114(1):1-14
[14]RINAUDO M.Chitin and chitosan: Properties and applications[J].Prog. Polym. Sci,2006,(31):603-632
[15]NO H K, MEYERS S P, PRINYAWIWATKUL W, et al.Applications of chitosan for improvement of quality and shelf life of foods: A review[J].J. of Food Sci,2007,(72):87-100
[16]李治,刘晓非, 杨冬芝, 管云林等.壳聚糖降解研究进展[J].化工进展,2000,(06):20-23
[17]JEYAMKONDAN S, JAYAS D S, HOLLEY R A.Pulsed electric field processing of foods: A review[J].J. Food Prot,1999,62(9):1088-1096
[18]HAN Zhong, ZENG Xin’an, YU Shujuan, et al.Effects of pulsed electric fields (PEF) treatment on physicochemical properties of potato starch[J].Innov. Food Sci. Emerg,2009,:481-485
[19]HAN Zhong ZENG Xin’an ZHANG Benshan.Effects of pulsed electric fields (PEF) treatment on the properties of corn starch[J].J. Food Eng,2009,:318-323
[20]WANG Wei, BO Shuqin, LI Shuqing, et al.Determination of the Mark–Houwink equation for chitosans with different degrees of deacetylation[J].Int. J. Biol. Macromol.,1991,13:281-285
[21]魏波, 骆仲泱, 徐飞等.脉冲电晕放电中OH自由基的发射光谱研究[J].光谱学与光谱分析,2010,(02):293-296
[22]黄熙, 李方, 朱孔营.H2O2氧化降解壳聚糖的动力学[J].天津理工大学学报,2005,(04):71-73
[23]RASO J, HEINZ V.Pulsed electric fields technology for the food industry: Fundamentals and applications[J].Springer,2006,:158-160
[24]张卫国, 周永国, 杨越冬等.有机酸及降解条件对壳聚糖降解速度的影响[J].河北科技师范学院学报,2006,(01):32-34
[25]SAITO H, TABETA R, OGAWA K.High-resolution solid-state 13C-NMR study of chitosan and its salts with acids[J].,1987,20:2424-2430