植物活性成分对细胞存活率的影响及其测定方法的比较研究

食品科学 ›› 2012, Vol. 33 ›› Issue (19): 119-123.

植物活性成分对细胞存活率的影响及其测定方法的比较研究

孙健，梅晶，赵晓红*，刘涛，阮研

北京联合大学应用文理学院生物活性物质与功能食品北京市重点实验室

收稿日期:2012-06-20 修回日期:2012-09-01 出版日期:2012-10-15 发布日期:2012-09-17
通讯作者: 赵晓红 E-mail:xiaohong@buu.edu.cn
基金资助:
北京市教育委员会科技计划重点项目

The Effects of Bioactive Plant Compounds on Cell viability and Comparison of Different Measuring Methods

Received:2012-06-20 Revised:2012-09-01 Online:2012-10-15 Published:2012-09-17
Contact: Xiao-Hong ZHAO E-mail:xiaohong@buu.edu.cn
Supported by:
the key item of Science & Technology Fund Planning Project of Beijing municipal commission of education

摘要/Abstract

摘要： 选用植物活性物质单体白藜芦醇和绿原酸作用于中国仓鼠卵巢(CHO)细胞，采用MTS法、WST-1法、CCK-8法、XTT法和MTT法测定细胞存活率，并将MTS法、WST-1法、CCK-8法、XTT法这4种方法与传统的MTT法进行比较分析，从中选出最佳的检测方法；通过IC50值比较白藜芦醇和绿原酸毒性大小。结果显示：CCK-8法所得结果稳定，且与MTT法所测得的IC50值最接近，为最佳选择；WST-1法的实验可重复性较差，结果偏小；XTT法是4种方法中最不稳定的，作用时间、检测波长最难确定，且实验重复性差。CCK-8法、MTS法、WST-1法、XTT法和MTT法测定所得白藜芦醇的IC50值分别是116.86、124.68、64.90、162.86、86.30μmol/L；绿原酸的IC50值分别为2808.60、1119.96、1029.72、1325.43、2852.63μmol/L，同种方法下绿原酸的IC50值均高于白藜芦醇的IC50值。结论是测定植物活性成分对细胞存活率影响的方法以CCK-8为最好。白藜芦醇对CHO细胞的毒性比绿原酸大。

关键词: MTS, WST-1, CCK-8, XTT, 细胞存活率, 白黎芦醇, 绿原酸

Abstract: Resveratrol and chlorogenic acid, as phytochemicals were used to treat CHO cells. Cell viability was measured by using MTS, WST-1, CCK-8, XTT and MTT methods and the optimal method from MTS, WST-1, CCK-8 and XTT was screened by comparing with the conventional MTT methods. The cytotoxicity of resveratrol and cholorogenic was compared by IC50 value. Results: Compared with MTT assay, CCK-8 assay was timesaving, repeatable and easily operated. IC50 of resveratrol and chlorogenic acid detected by CCK-8 were closed to one by MTT assay. Thus, CCK-8 assay was optimal. On the contrary, WST-1 assay was less repeatable and cell viability rate tended to smaller. XTT assay was the worst because of its instability in wavelength, experimental time and repetition. IC50 of resveratrol on CHO cells were 116.86、124.68、64.90、329.83、86.30 μmol/L in CCK-8、MTS、WST-1、XTT and MTT assay. While IC50 of chlorogenic acid on cells were 2808.60、1119.96、1029.72、1325.43、2852.63 μmol/L respectively. The results showed higher IC50 value for chlorogenic acid. Conclusions: CCK-8 assay was a optimal method for cell viability ; Resveratrol showed higher cytotoxicity to CHO cells compared with chlorogenic acid.

中图分类号:

Q946
R994.4

孙?健，梅?晶，赵晓红*，刘?涛，阮研. 植物活性成分对细胞存活率的影响及其测定方法的比较研究[J]. 食品科学, 2012, 33(19): 119-123.

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