食品科学 ›› 2012, Vol. 33 ›› Issue (19): 273-277.

• 营养卫生 • 上一篇    下一篇

罗丹明B在金鱼(Carassius auratus)组织中的蓄积及消除规律

纪淑娟,王 莎,李玲楠,檀德宏   

  1. 沈阳农业大学食品学院
  • 收稿日期:2012-06-20 修回日期:2012-09-07 出版日期:2012-10-15 发布日期:2012-09-17
  • 通讯作者: 纪淑娟 E-mail:syaujsj@163.com
  • 基金资助:

    “十二五”国家科技支撑计划重点项目

Accumulation and Elimination of Rhodamine B in Carassius auratus Tissues

  • Received:2012-06-20 Revised:2012-09-07 Online:2012-10-15 Published:2012-09-17

摘要: 采用水体暴露方法,研究金鱼(Carassius auratus)肝脏、肾脏、腮、肌肉和椎骨对不同质量浓度(5mg/L和15mg/L) 罗丹明B的蓄积规律,采用注射染毒然后在清水中饲养的方法研究鱼体组织罗丹明B的消除规律。结果表明:罗丹明B质量浓度为5mg/L和15mg/L时,分别在第15天和12天达到吸收平衡。蓄积达到平衡时罗丹明B在高、低染毒质量浓度中的最大和最小蓄积系数分别出现在肝脏和肌肉,最大蓄积系数分别为16.14和7.37,最小蓄积系数分别为0.97和0.77。注射染毒后罗丹明B消除研究结果表明:随时间延长罗丹明组织浓度逐步下降,第9天接近平衡。不同组织中消除速度表现:肾脏>肌肉>椎骨>腮>肝脏。生物半衰期t1/2在组织中的差异表现为:肝脏>腮>椎骨>肾脏>肌肉。

关键词: 罗丹明B, 金鱼, 蓄积, 消除, 生物蓄积系数

Abstract: Goldfishes (Carassius auratus) were exposed to rhodamine B at concentrations of 5 mg/L and 15 mg/L in water to explore the accumulation of rhodamine B in the liver, kidney, cheek, muscle and spine. Meanwhile, additional goldfishes were intraperitoneally injected with rhodamine B and placed back into dechlorinated tap water to investigate the elimination of rhodamine B. Rhodamine B reached an equilibrium state in goldfishes after 15 days and 12 days of absorption at 5 mg/L and 15 mg/L. The maximum and minimum accumulation coefficients of rhodamine B for equilibrium absorption at the high and low concentrations were observed in the liver and muscle, respectively, which were 16.14 and 7.37 for maximum accumulation, and were 0.97 and 0.77 for minimum accumulation, respectively. The concentrations of rhodamine B in goldfish organs were gradually eliminated and reached equilibrium on the 9th day after intraperitoneal injection. Different goldfish tissues could be ranked as follows: kidney > muscle > bone > gill > liver in decreasing order of the elimination rate of rhodamine B, and liver > gill > bone > kidney > muscle in decreasing order of the half-life of rhodamine B.

Key words: rhodamine B, Carassius auratus, accumulation, elimination, bioaccumulation coefficient

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