直接竞争化学发光酶免疫法检测谷物中的伏马菌素B1

食品科学 ›› 2012, Vol. 33 ›› Issue (22): 173-176.

直接竞争化学发光酶免疫法检测谷物中的伏马菌素B1

何庆华1,2，许杨1,2,*，刘师文2

1.食品科学与技术国家重点实验室 2.南昌大学中德联合研究院

收稿日期:2012-06-29 修回日期:2012-10-16 出版日期:2012-11-25 发布日期:2012-11-20
通讯作者: 何庆华 E-mail:heqinghua@ncu.edu.cn
基金资助:
国家自然科学基金面上项目;食品科学与技术国家重点实验室自由探索项目

Determination of Fumonisin B1 in Cereal Samples by Direct Competitive Chemiluminescene Enzyme Immunoassay

Received:2012-06-29 Revised:2012-10-16 Online:2012-11-25 Published:2012-11-20
Contact: HE Qing-Hua E-mail:heqinghua@ncu.edu.cn

摘要/Abstract

摘要： 建立1种检测谷物中伏马菌素B1 的直接竞争化学发光酶免疫方法。该方法的最佳检测条件为：抗原包被质量浓度0.4μg/mL，酶标抗体3000倍稀释，竞争反应时间20min；检测限为0.13ng/mL，半抑制浓度(IC50)为1.43ng/mL，批内变异系数2.4%，批间变异系数9.2%，以玉米为样品的加标回收率达到100.2%～115.4%。谷物盲样的检测结果显示，该方法与酶联免疫吸附检测试剂盒、高效液相色谱检测结果的相关系数分别为0.9793、0.9851，三者之间无显著性差异，所建立的直接竞争化学发光酶免疫方法可用于谷物样品中伏马菌素B1的大规模快速筛查。

关键词: 伏马菌素B1, 化学发光酶免疫分析, 直接竞争酶联免疫分析

Abstract: A direct competitive chemiluminescene enzyme immunoassay (dcCL-EIA) was developed for the rapid determination of fumonisin B1 in cereal samples. Assay parameters including coating antigen (FB1-OVA) and enzyme labeled anti-FB1 antibodies and reaction time were optimized to be 0.4 μg/mL, 3000-fold dilution and 20 min, respectively. The analytical sensitivity of the developed method was 0.13 ng/mL, and the IC50, intra-assay and inter-assay coefficients of variation were 1.43 ng/mL, 2.4% and 9.2%, respectively. The recovery rates for FB1 in negative corn samples at three levels were in the range of 100.2%～115.4%. A total of 40 cereal samples were screened by DC-CLEIA, ELISA kit and HPLC, respectively. The results indicated that the correlation coefficients (R2) for the results from ELISA kit and HPLC were 0.9793 and 0.9851, respectively. The dcCL-EIA method is sensitive, rapid and accurate, and is suitable for the large-scale screening of FB1 in cereal samples.

Key words: fumonisin B1, chemiluminescene enzyme immunoassay, direct competitive ELISA

中图分类号:

TS207.3

何庆华1,2，许?杨1,2,*，刘师文2. 直接竞争化学发光酶免疫法检测谷物中的伏马菌素B1[J]. 食品科学, 2012, 33(22): 173-176.

[1]	宁霄，张伟清，梁瑞强，曹进，张庆生. 3 种β-兴奋剂化学发光酶免疫试剂盒分析检测方法的评价[J]. 食品科学, 2016, 37(12): 238-242.
[2]	王少康，王婷婷，黄桂玲，石若夫，杨立刚，董淑楠，孙桂菊*. 伏马菌素B1对人正常食管上皮细胞细胞周期相关基因mRNA表达的影响[J]. 食品科学, 2013, 34(23): 313-316.
[3]	邱云青，王伟，李凤琴*. 化学发光酶免疫分析法检测食品中赭曲霉毒素[J]. 食品科学, 2010, 31(24 ): 432-435.
[4]	刘师文，何庆华，邹龙，许杨*. 谷物中伏马菌素B1酶联免疫分析法的建立[J]. 食品科学, 2010, 31(18): 350-354.