产α-葡萄糖苷酶抑制剂菌株的筛选及发酵条件优化

食品科学 ›› 2012, Vol. 33 ›› Issue (23): 249-253.

产α-葡萄糖苷酶抑制剂菌株的筛选及发酵条件优化

李娟，刘兰，于配配，黄梅华，张云开，陈桂光，梁智群*

广西大学生命科学与技术学院

收稿日期:2011-09-30 修回日期:2012-11-03 出版日期:2012-12-15 发布日期:2012-12-12
通讯作者: 梁智群 E-mail:zqliang@gxu.edu.cn

Screening of α-Glucosidase Inhibitor-producing Strain and Optimization of Fermentation Conditions

Received:2011-09-30 Revised:2012-11-03 Online:2012-12-15 Published:2012-12-12

摘要/Abstract

摘要： 从农田土壤中筛选得到一株产α-葡萄糖苷酶抑制剂能力较强的UN-8菌株，菌株对其发酵条件进行初步优化，得到最优条件为玉米粉3g/100mL、酵母膏1.2g/100mL、溶氧量30mL/250mL、摇床转速160r/min、发酵时间3d、初始pH 8.0以及培养温度32℃。在此条件下，α-葡萄糖苷酶抑制剂的抑制率为75.34%，比初始抑制率(60.79%)提高了14.55%。

关键词: α-葡萄糖苷酶抑制剂, 筛选, 发酵条件, 优化, 提高

Abstract: A high-yield α-glucosidase inhibitor-producing strain named as UN-8 was isolated from farm soil and fermentation conditions for the production of α-glucosidase inhibitor by UN-8 were optimized. The optimal fermentation conditions were determined as follows: 3 g/100 mL of corn meal, 1.2 g/100 mL of yeast extract, 30 mL of fermentation medium in a 250-mL shake flask, 160 r/min of shaking speed, initial pH 8.0 and 32 ℃ of fermentation temperature. The inhibitory rate of α-glucosidase inhibitor produced under these conditions was 75.34%, which was 14.55% higher than before the optimization (60.79%).

Key words: α-glucosidase inhibitor, screening, fermentation condition, optimization, improvement

中图分类号:

Q939.97

李?娟，刘?兰，于配配，黄梅华，张云开，陈桂光，梁智群*. 产α-葡萄糖苷酶抑制剂菌株的筛选及发酵条件优化[J]. 食品科学, 2012, 33(23): 249-253.

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