食品科学 ›› 2013, Vol. 34 ›› Issue (10): 327-330.doi: 10.7506/spkx1002-6630-201310072

• 技术应用 • 上一篇    

真空气流细胞破壁技术对桑叶中有效成分提取的影响

孙长波,石磊岭,涂建飞,韩丹丹,张清清,张 晶   

  1. 1.吉林农业大学中药材学院,吉林 长春 130118;2.吉林农业大学发展学院,吉林 长春 130600;
    3.新疆中药民族药研究所,新疆 乌鲁木齐 830002
  • 收稿日期:2012-04-11 修回日期:2013-03-22 出版日期:2013-05-25 发布日期:2013-05-07
  • 通讯作者: 张晶 E-mail:zhjing0701@163.com

Effect of Vacuum Air Current Technique for Plant cell Wall Breakdown (VAPB) on the Extraction of Active Ingredients in Mulberry Leaves

SUN Chang-bo,SHI Lei-ling,TU Jian-fei,HAN Dan-dan,ZHANG Qing-qing,ZHANG Jing   

  1. 1. College of Chinese Medicine Material, Jilin Agricultural University, Changchun 130118, China;
    2. Development College, Jilin Agricultural University, Changchun 130600, China;
    3. Xinjiang Institute of Chinese Materia Medicao and Ethical Materia Medica, Ürümqi 830002, China
  • Received:2012-04-11 Revised:2013-03-22 Online:2013-05-25 Published:2013-05-07
  • Contact: Jing ZHANG E-mail:zhjing0701@163.com

摘要:

目的:研究真空气流细胞破壁技术(VAPB)对桑叶中生物碱、黄酮及多糖提取率的影响。方法:采用HPLC法,分别以1-脱氧野尻霉素及芦丁为对照品,测定破壁与未破壁桑叶中的生物碱及黄酮含量;采用分光光度法,以葡萄糖为对照品,测定了破壁与未破壁桑叶中的多糖的含量,并比较测定值。结果:破壁较未破壁桑叶样品中的生物碱、黄酮及多糖含量的测定值分别高出74%、58%和48%。结论:采用VAPB对桑叶进行提取前处理,可提高其有效成分的溶出率,更好地发挥其生物活性。

关键词: 桑叶, 真空气流细胞破壁, 生物碱, 黄酮, 多糖, 提取率

Abstract:

Objective: To investigate the effect of VAPB on the extraction efficacy of alkaloids, flavonoids and
polysaccharides in mulberry leaves. Methods: The contents of alkaloids, flavonoids and polysaccharides in mulberry leaves
with damaged cell wall and intact wall were determined and compared by HPLC or spectrophotometric methods. Results:
The contents of alkaloids, flavonoids and polysaccharides in mulberry leaf samples with damaged cell wall were higher than
those with intact cell wall, which were 74%, 58% and 48% respectively. Conclusions: Pretreatment of mulberry leaves by
VAPB may significantly increase the solubility of the active components in mulberry leaves.

Key words: mulberry leaves, vacuum air current for plant cell wall breakdown, alkaloid, flavonoid, polysaccharide, extraction

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