• 生物工程 •

### 不同pH值酿酒酵母分批发酵生产谷胱甘肽数学模型建立

1. 1.常熟理工学院生物与食品工程学院，江苏 常熟 215500；2.苏州市食品生物技术重点实验室，江苏 常熟 215500
• 收稿日期:2012-03-31 修回日期:2013-05-14 出版日期:2013-07-15 发布日期:2013-06-28
• 通讯作者: 齐斌 E-mail:qibin65@126.com
• 基金资助:

国家自然科学基金面上项目(31171758)；2011年度常熟理工学院“青蓝工程”优秀青年骨干教师培养对象项目

### Mathematical Modeling of Glutathione Production by Saccharomyces cerevisiae in Batch Fermentation at Different pH

ZHENG Li-xue1,2，XIE Qun-fan1，WANG Li-mei1,2，QI Bin1,*

1. 1. College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China；
2. Suzhou Key Laboratory of Food and Biotechnology, Changshu 215500, China
• Received:2012-03-31 Revised:2013-05-14 Online:2013-07-15 Published:2013-06-28
• Contact: QI Bin E-mail:qibin65@126.com

Abstract:

A mathematical model of glutathione production by Saccharomyces cerevisiae Y518 in batch fermentation at
different pH was established with malt sprout extract as the fermentation medium. The results showed that Gaussian fitting
model was in good agreement with the experimental observations for glutathione production at different pH, with correlation
coefficient (R2) of 0.99235 (pH5.0) and 0.98842 (pH6.0), but not suitable for biomass and substrate consumption prediction.
Glutathione production reached the maximum of 72 mg/L at 32 h yielding a biomass of 4.59 g/L when pH was kept constant
at 5.0. In contrast, maximum glutathione production of 91 mg/L was also obtained at 32 h along with a biomass of 4.11 g/L
when pH was kept constant at 6.0.