食品科学 ›› 2013, Vol. 34 ›› Issue (4): 168-172.

• 分析检测 • 上一篇    下一篇

超高效液相色谱法同时测定柑桔中11种类黄酮物质

冉玥1,焦必宁2,赵其阳2   

  1. 1. 西南大学食品科学学院
    2. 西南大学食品科学学院;中国农业科学院柑桔研究所
  • 收稿日期:2012-03-30 修回日期:2013-01-11 出版日期:2013-02-25 发布日期:2013-01-29
  • 通讯作者: 焦必宁 E-mail:bljiao@tom.com
  • 基金资助:

    农业部现代农业(柑橘)产业技术体系建设专项;农业948项目

Stimultaneous Determination of 11 Flavonoids in Citrus by Ultra Performance Liquid Chromatography

  • Received:2012-03-30 Revised:2013-01-11 Online:2013-02-25 Published:2013-01-29
  • Contact: Bining Jiao E-mail:bljiao@tom.com

摘要:

通过正交实验优化了样品前处理条件,建立了柑桔中11种类黄酮物质的超高效液相色谱-二极管阵列检测器(UPLC-PAD)分析法。以甲醇-乙酸水溶液为流动相,梯度洗脱,8种黄烷酮和3种多甲氧基黄酮11分钟内完全基线分离(检测波长283nm和330nm)。方法检出限为0.005~0.02 mg/kg(S/N=3),线性方程的相关系数均达到0.9991以上。果皮和果汁的回收率分别为94.6%~101.2%,94.5%~101.8%;相对标准偏差分别为1.1%~3.7%,0.9%~3.9%(n=6)。该方法快速、高效、低成本、环境友好、结果准确可靠,能满足柑桔中类黄酮的检测要求。

Abstract:

A rapid method for qualitative and quantitative analysis of 11 flavonoids in citrus was developed using ultra performance liquid chromatography (UPLC) coupled with photodiode array detector. Under the condition of methanol-.acetic acid water solution as mobile phase and gradient elution, 8 flavanones and 3 polymethacrylate flavones were separated to baseline within 11 min. The detection wavelengthes were 283nm and 330nm. The limits of detection (LODs) were from 0.005 to 0.02mg/kg (S/N=3) and good linearity was observed with the correlation coefficient (r2)≥0.9991.The influencing factors sample preparation were optimized by using Orthogonal experimental design and the recoveries of compounds analyzed from peel and juice samples were in the range of 94.6–101.2% with RSD between 1.1 and 3.7%, and in the range of 94.5–101.8% with RSD between 0.9 and 3.9%, respectively.Compared with the existing, this method was more effective, environmentally friendly and reproducible.

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