关键词: 分散固相萃取, 气相色谱-电子捕获检测法, 水产品, 氯霉素类药物

Abstract:

An efficient, sensitive, and low matrix interference method for the determination of chloramphenicol (CAP) and
florfenicol (FF) in fishery products using gas chromatography-electron capture detector (GC-ECD) has been developed.
Samples were treated by extraction with ethyl acetate and n-hexane purification. Further cleanup of the extracts was
processed by a modified dispersive solid phase extraction procedure. Then, the purified solutions were derivatized with slilyl
reagents. The linearity of the method ranged from 1.5 to 100 μg/L for CAP, and from 6 to 400 μg/L for FF, with correlation
coefficients ranging between 0.998 1 and 0.999 7. The limits of detection were 0.1–0.3 μg/kg. The recoveries of spiked CAP
and FF with external calibration method at different concentration levels in different sample matrixes (crucian, blue crab,
and Penaeus vannawei) were 82%–106%, 87%–111%, and 91%–98%, respectively, with relative standard deviations of
2.3%–4.9%, 2.4%–4.5%, and 1.4%–4.1% (n = 5), respectively. Thus it is concluded that this method can be successfully
applied for the determination of choramphenicols (CAPs) in fishery products.

Key words: dispersive solid phase extraction, gas chromatography-electron capture detector, fisher products, choramphenicols