食品科学

• 安全检测 • 上一篇    下一篇

生鲜猪肉低场核磁检测参数优化

王志永,王 欣*,夏义苗,黄远芬,刘宝林   

  1. 上海理工大学食品质量与安全研究所,上海 200093
  • 出版日期:2015-06-25 发布日期:2015-06-12
  • 通讯作者: 王 欣
  • 基金资助:

    国家自然科学基金青年科学基金项目(NSFC31201365);上海市科学技术委员会重点攻关项目(11142200403)

Assessment of Fresh Pork Quality by LF-NMR: Parameter Optimization

WANG Zhiyong, WANG Xin*, XIA Yimiao, HUANG Yuanfen, LIU Baolin   

  1. Institute of Food Safety and Quality, University of Shanghai for Science and Technology, Shanghai 200093, China
  • Online:2015-06-25 Published:2015-06-12
  • Contact: WANG Xin

摘要:

低场核磁共振(low field nuclear magnetic resonance,LF-NMR)技术在反映肉品内部信息上具有显著优势,而合适的检测参数对于测定结果的准确性提高检测效率至关重要。以生鲜猪精瘦肉为对象,对其LF-NMR检测中的检测参数,如重复采样等待时间、半回波时间、回波个数、重复扫描次数、样品质量及温度等进行优化,并进行了验证。结果表明,取质量4~5 g的样品于32 ℃水浴至恒温后,在重复采样等待时间4 500 ms、半回波时间200 μs、回波个数3 000、重复扫描次数8、采样频率250 kHz的条件下采样,有利于获得良好稳定性与重复性的检测结果。所得条件可用于猪肉的LF-NMR相关研究,亦可为其他肉制品在LF-NMR中的研究提供借鉴。

关键词: 猪肉, 低场核磁共振, 参数优化

Abstract:

Information about water mobility and distribution in meat can be provided by proton nuclear magnetic resonance
(NMR) relaxometry. The suitable measurement parameters of low-field proton nuclear magnetic resonance (LF-NMR)
relaxometry will not only be helpful for the reliability of experiment data, which reflect accurately the information on the
sample, but also be beneficial for enhancing the detection efficiency. In this study, using fresh lean pork from the hind
legs, the effects of LF-NMR parameters on the accuracy, stability and reproducibility of the experimental results were
investigated. No significant changes in experimental data (P > 0.05) were observed when the tR (repetition delay) and Echo
Count (sampling data number) were set as more than 4 500 ms and more than 3 000, respectively. The optimal conditions
were determine as follows: τ value (time between 90 degree pulse and 180 degree pulse) of 200 μs, N of 8 and SW value
(receiver bandwidth) of 250 kHz during the test. Good stability and reproducibility could be guaranteed when the sample
was cut into lumps (about 4–5 g), incubated in a water bath at 32 ℃, and subsequently transferred to the probe before the
measurement. These results can provide appropriate detection conditions for applications of LF-NMR for quality assessment
of fresh lean pork and other meats.

Key words: pork, low field-nuclear magnetic resonance, optimal parameters

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