食品科学 ›› 2018, Vol. 39 ›› Issue (10): 242-249.doi: 10.7506/spkx1002-6630-201810037

• 成分分析 • 上一篇    下一篇

基于OAV和AEDA对工夫红茶的PLSR分析

肖作兵1,2,王红玲1,牛云蔚1,*,朱建才1,马宁1   

  1. (1.上海应用技术大学香料香精技术与工程学院,上海 201418;2.上海香料研究所,上海 200232)
  • 出版日期:2018-05-25 发布日期:2018-05-15
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2016YFA0200304);上海香料香精工程技术研究中心能力提升项目(15DZ2280100)

Analysis of Aroma Components in Four Chinese Congou Black Teas by Odor Active Values and Aroma Extract Dilution Analysis Coupled with Partial Least Squares Regression

XIAO Zuobing1,2, WANG Hongling1, NIU Yunwei1,*, ZHU Jiancai1, MA Ning1   

  1. (1. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China;2. Shanghai Research Institute of Fragrance and Flavor Industry, Shanghai 200232, China)
  • Online:2018-05-25 Published:2018-05-15

摘要: 采用顶空蒸汽蒸馏提取结合气相色谱-质谱联用技术及气相色谱-嗅闻技术,对4?种中国工夫红茶进行定性、定量分析。根据香气成分的香气活力值和芳香萃取物稀释分析分别筛选出30、36?种挥发性成分作为香气成分,结合2?种方法确定22?种香气成分为工夫红茶的香气关键成分,并将其与感官属性和红茶样品进行偏最小二乘法相关性分析。确定对工夫红茶香气起重要作用的22?种关键香气物质为:1-戊醇、叶醇、1-辛烯-3-醇、反式氧化芳樟醇、芳樟醇、顺-6-壬烯醇、香叶醇、苯甲醇、苯乙醇、橙花叔醇、戊醛、己醛、反-2-己烯醛、糠醛、(反,反)-2,4-庚二烯醛、苯乙醛、(反,反)-2,4-癸二烯醛、水杨酸甲酯、丙位-壬内酯、2,3-丁二酮、2-庚酮和香豆素。

关键词: 中国工夫红茶, 香气成分, 顶空蒸汽蒸馏提取, 气相色谱-质谱联用, 气相色谱-嗅觉测量法, 香气活力值, 芳香萃取物稀释分析, 偏最小二乘回归法

Abstract: The volatile components of four Chinese congou black teas were qualitatively and quantitatively investigated by headspace steam distillation extraction (HSDE) followed by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-olfactometry (GC-O). Thirty, thirty six and twenty two volatile components were identified as aroma-active compounds by odor active values (OAV), aroma extract dilution analysis (AEDA) and their combination, respectively. The correlations between sensory attributes and key aroma-active compounds were modeled by partial least squares regression (PLSR). Twenty two key aroma-active compounds were found to make an important contribution to the aroma of Chinese congou black tea, including 1-pentanol, cis-3-hexen-1-ol, 1-octen-3-ol, trans-linalool oxide, linalool, cis-6-nonenol, geraniol, benzyl alcohol, phenylethyl alcohol, nerolidol, valeraldehyde, hexanal, trans-2-hexenal, furfural, (E,E) -2,4-heptadienal, phenylacetaldehyde, (E,E)-2,4-decedienal, methyl salicylate, γ-nonalactone, 2,3-butanedione, 2-heptanone, and coumarin.

Key words: Chinese congou black tea, aroma components, headspace steam distillation extraction (HSDE), gas chromatography-mass spectrometry (GC-MS), gas chromatography-olfactometry (GC-O), odor active values (OAV), aroma extract dilution analysis (AEDA), partial least squares regression (PLSR)

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