饲养方式对苏尼特羊肌内脂肪沉积途径AMPK-ACC-CPT1通路和肉品品质的影响

doi:10.7506/spkx1002-6630-20171107-076

食品科学 ›› 2019, Vol. 40 ›› Issue (1): 31-36.doi: 10.7506/spkx1002-6630-20171107-076

饲养方式对苏尼特羊肌内脂肪沉积途径AMPK-ACC-CPT1通路和肉品品质的影响

袁倩，王宇，苏琳，罗玉龙，苏日娜，赵丽华，靳烨*

内蒙古农业大学食品科学与工程学院，内蒙古呼和浩特 010018

出版日期:2019-01-15 发布日期:2019-01-22
基金资助:
国家自然科学基金地区科学基金项目（31660439）；“十三五”国家重点研发计划重点专项（2016YFE0106200）

Effects of Different Feeding Systems on Intramuscular Fat Deposition Pathway AMPK-ACC-CPT1 and Meat Quality in Sunit Lamb

YUAN Qian, WANG Yu, SU Lin, LUO Yulong, SU Rina, ZHAO Lihua, JIN Ye*

College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China

Online:2019-01-15 Published:2019-01-22

摘要/Abstract

摘要： 以放牧和舍饲两种饲养条件下12 月龄苏尼特羊股二头肌为实验材料，利用酶联免疫吸附测定法和实时荧光定量聚合酶链式反应法对肌内脂肪沉积动态平衡中脂肪酸β氧化一磷酸腺苷激活蛋白激酶（AMP-activated protein kinase，AMPK）-乙酰辅酶A羧化酶（acetyl-CoA carboxylase，ACC）-肉毒碱脂酰转移酶1（carnitine palmitoyltransferase，CPT1）通路所涉及的相关酶活力和质量浓度、相关基因mRNA表达量及肉品品质等指标进行测定，研究不同饲养方式对AMPK-ACC-CPT1信号通路相关指标、肌内脂肪含量及肉品品质产生的影响。结果表明：放牧饲养组羊股二头肌AMPK质量浓度、AMPK活力、CPT1质量浓度、AMPK mRNA表达量、亮度、黄度和剪切力显著大于舍饲饲养组（P＜0.05）；放牧饲养组羊股二头肌ACC活力、ACC mRNA表达量、肌内脂肪含量显著低于舍饲饲养组（P＜0.05）。由此可见，放牧饲养可以一定程度上激活AMPK-ACC-CPT1通路，从而减少肌内脂肪沉积，增加羊肉亮度和黄度，降低嫩度，进而影响肉品品质。

关键词: 饲养方式, 苏尼特羊, 一磷酸腺苷激活蛋白激酶, 脂肪酸β氧化, 肉品品质

Abstract: In the present study, we assessed the effects of two different feeding systems on the activities and mRNA expression levels of enzymes involved in the AMP-activated protein kinase-acetyl-CoA carboxylase-carnitine palmitoyltransferase (AMPK-ACC-CPT1) pathway, fat content and meat quality in the Biceps femoris muscle of 12-month-old Sunit lambs. The enzyme activities were determined by enzyme-linked immunosorbent assay and gene expression was measured by real-time fluorescence quantitative polymerase chain reaction (PCR). The results showed that AMPK concentration and activity, AMPK mRNA expression, CPT1 concentration, brightness (L* value), yellowness (b* value) and shear force in the biceps femoris muscle of grazed lambs were significantly higher than those in stall-fed lambs (P < 0.05), while the opposite was observed for ACC activity, ACC mRNA expression and intramuscular fat content (P < 0.05). Accordingly we concluded that grazing can activate the AMPK-ACC-CPT1 pathway to reduce intramuscular fat deposition, increase L* and b* values and decrease tenderness, consequently affecting lamb meat quality.

Key words: feeding systems, Sunit lamb, AMP-activated protein kinase, fatty acid β oxidation, meat quality

中图分类号:

TS251.6

袁倩，王宇，苏琳，罗玉龙，苏日娜，赵丽华，靳烨. 饲养方式对苏尼特羊肌内脂肪沉积途径AMPK-ACC-CPT1通路和肉品品质的影响[J]. 食品科学, 2019, 40(1): 31-36.

YUAN Qian, WANG Yu, SU Lin, LUO Yulong, SU Rina, ZHAO Lihua, JIN Ye. Effects of Different Feeding Systems on Intramuscular Fat Deposition Pathway AMPK-ACC-CPT1 and Meat Quality in Sunit Lamb[J]. FOOD SCIENCE, 2019, 40(1): 31-36.

[1]	侯艳茹，苏琳，侯普馨，白艳苹，孙冰，赵丽华，黄凯飚，吕科，靳烨. 饲养方式对苏尼特羊肌纤维组成和肉品质的影响及其调控机理[J]. 食品科学, 2021, 42(7): 83-89.
[2]	李文博，罗玉龙，郭月英，刘畅，窦露，赵丽华，苏琳，靳烨. 苏尼特羊宰后成熟过程中肌原纤维蛋白特性与肉品质的变化分析[J]. 食品科学, 2021, 42(3): 48-55.
[3]	窦露，刘畅，巴吉木色，杨致昊，苏琳，赵丽华，田建军，郭月英，靳烨. 日粮添加乳酸菌对苏尼特羊生长、肉品质、风味物质和抗氧化能力的影响[J]. 食品科学, 2021, 42(1): 25-32.
[4]	谭子璇, 柏雪, 罗璠, 郭琼, 刘欣宇, 冉黎, 高彦华, . 不同饲养方式对牦牛瘤胃微生物区系及肌肉品质的影响[J]. 食品科学, 2020, 41(15): 79-87.
[5]	王芳芳，张一敏，罗欣，梁荣蓉，毛衍伟. 冷冻解冻对生鲜肉品质的影响及其新技术研究进展[J]. 食品科学, 2020, 41(11): 295-302.
[6]	杜瑞，靳烨，王柏辉，罗玉龙，宝勒格，赵丽华，苏琳. 益生菌对苏尼特羊胃肠道菌群、代谢物及肉品质的影响[J]. 食品科学, 2020, 41(11): 14-21.
[7]	侯普馨，侯艳茹，白艳苹，苏琳，赵丽华，宝勒格，靳烨. 日粮添加亚麻籽对苏尼特羊肌纤维特性及肉品质的影响[J]. 食品科学, 2020, 41(11): 36-42.
[8]	袁倩，王柏辉，苏琳，要铎，王德宝，赵丽华，靳烨. 两种饲养方式对苏尼特羊肉脂肪酸组成和脂肪代谢相关基因表达的影响[J]. 食品科学, 2019, 40(9): 29-34.
[9]	李文博，罗玉龙，刘畅，窦露，赵丽华，苏琳，靳烨. 饲养方式对苏尼特羊肉挥发性风味成分和脂肪酸组成的影响[J]. 食品科学, 2019, 40(24): 207-213.
[10]	罗玉龙，刘畅，李文博，王柏辉，窦露，杜瑞，要铎，赵丽华，苏琳，靳烨. 两种饲养方式下苏尼特羊肉的氧化稳定性[J]. 食品科学, 2019, 40(17): 30-35.
[11]	罗玉龙，刘畅，李文博，王柏辉，窦露，赵丽华，杜瑞，王政纲，靳烨. 两种饲养方式下苏尼特羊肉中鲜味物质含量及相关调控基因表达量[J]. 食品科学, 2019, 40(13): 8-13.
[12]	侯普馨，侯艳茹，白艳苹，苏琳，赵丽华，靳烨. Ca2＋信号通路调控肌纤维类型转化的研究进展[J]. 食品科学, 2019, 40(11): 289-294.
[13]	李墨琳，罗欣，刘国星，毛衍伟，张一敏. 活性包装对肉制品品质及货架期影响的研究进展[J]. 食品科学, 2019, 40(11): 313-320.
[14]	罗玉龙，王柏辉，赵丽华，苏琳，苏日娜，侯艳茹，杨蕾，靳烨. 苏尼特羊和小尾寒羊的屠宰性能、肉品质、脂肪酸和挥发性风味物质比较[J]. 食品科学, 2018, 39(8): 103-107.
[15]	王柏辉，杨蕾，苏日娜，罗玉龙，王宇，赵丽华，靳烨. 饲养方式对苏尼特羊屠宰性能、羊肉品质及脂质氧化性能的影响[J]. 食品科学, 2018, 39(23): 41-46.

饲养方式对苏尼特羊肌内脂肪沉积途径AMPK-ACC-CPT1通路和肉品品质的影响

Effects of Different Feeding Systems on Intramuscular Fat Deposition Pathway AMPK-ACC-CPT1 and Meat Quality in Sunit Lamb

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价