食品科学 ›› 2019, Vol. 40 ›› Issue (14): 249-258.doi: 10.7506/spkx1002-6630-20180821-218

• 成分分析 • 上一篇    下一篇

基于UFLC-Triple TOF MS/MS技术分析竹节参中皂苷类成分

陈佳丽,谈梦霞,邹立思,刘训红,陈舒妤,石婧婧,王程成,梅余琪   

  1. 南京中医药大学药学院,江苏 南京 210023
  • 出版日期:2019-07-25 发布日期:2019-07-23
  • 基金资助:
    江苏高校优势学科建设工程资助项目(YSXK-2014);江苏高校品牌专业建设工程资助项目(PPZY2015A070)

Saponins in Panacis japonici Rhizoma as Analyzed by UFLC-Triple TOF MS/MS

CHEN Jiali, TAN Mengxia, ZOU Lisi, LIU Xunhong, CHEN Shuyu, SHI Jingjing, WANG Chengcheng, MEI Yuqi   

  1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
  • Online:2019-07-25 Published:2019-07-23

摘要: 采用超快速液相色谱-三重四极杆飞行时间质谱技术对竹节参中皂苷类成分进行分析。采用色谱柱SynergiTM Hydro-RP 100 ?(2.0 mm×100 mm,2.5 μm),以0.1%甲酸-水(A)-0.1%甲酸-乙腈(B)为流动相进行梯度洗脱;在电喷雾负离子扫描模式下采集数据。根据高分辨质谱提供的准分子离子和碎片离子的精确分子质量信息,结合标准品对照与参考相关文献数据,初步鉴定出53 种皂苷,包括18 种原人参二醇型、21 种原人参三醇型、10 种齐墩果烷型和4 种奥寇梯木醇型皂苷。本实验可为探索竹节参药材的药效物质基础和建立其药材品质的综合评价体系提供基础资料。

关键词: 竹节参, 超快速液相色谱-三重四极杆飞行时间质谱法, 皂苷

Abstract: In this study, we analyzed the saponins of Panacis japonici Rhizoma by ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF MS/MS). The chromatographic separation was performed on a SynergiTM Hydro-RP 100 ? (2.0 mm × 100 mm, 2.5 μm) with gradient elution using a mobile phase comprised of 0.1% formic acid solution (A)-0.1% formic acid acetonitrile (B). MS data were collected in the negative ion mode. Based on the accurate molecular mass information of quasi-molecular and fragment ions provided by highresolution mass spectrometry combined with standard reference data and relevant literature data, a total of 53 saponins were finally identified, including 18 glycosides of protopanaxadiol (PPD), 21 glycosides of protopanaxatriol (PPT), 10 glycosides of oleanolic acid and 4 glycosides of ocotillone. This study paves the way for exploring the pharmacodynamic material basis of P. japonici Rhizoma and developing a comprehensive quality evaluation system.

Key words: Panacis japonici Rhizoma, UFLC-Triple TOF MS/MS, saponins

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