关键词: 功能性肽, 内源性功能肽, 外源性功能肽, 富集, 分离

Abstract: Functional peptides are widely found in nature. The naturally occurring peptides in the human body are referred to as endogenous peptides, which are closely related to the physiological activities such as growth, metabolism, immunity, and signal transduction of living organisms. Exogenous peptides, with various functions such as anti-tumor, blood pressure lowering, and bone health-promoting effects, can be obtained by protein degradation or synthesized from amino acids. However, endogenous peptides are mostly present in low abundance, and are highly susceptible to high-abundance proteins during enrichment; exogenous peptides are mixed with other components. Therefore, the isolation of exogenous peptides and the enrichment of endogenous peptides are prerequisites for studying their structures and functions. In this paper, the current methods available to separate exogenous peptides and enrich endogenous peptides are reviewed, focusing on their principles, applicable objects, advantages and disadvantages as well as future directions. We hope that this paper will lay a theoretical foundation for the structural analysis and functional development of functional peptides.

Key words: functional peptide, endogenous peptide, exogenous peptide, enrichment, separation