食品科学 ›› 2020, Vol. 41 ›› Issue (20): 14-19.doi: 10.7506/spkx1002-6630-20190617-181

• 食品化学 • 上一篇    下一篇

脂肪含量对牦牛乳硬质干酪质构、流变和微观结构的影响

石永祺,梁琪,宋雪梅,张炎   

  1. (甘肃农业大学食品科学与工程学院,甘肃省功能乳品工程实验室,甘肃 兰州 730070)
  • 出版日期:2020-10-25 发布日期:2020-10-23
  • 基金资助:
    国家自然科学基金地区科学基金项目(31660468)

Effect of Fat Content on Texture, Rheology and Microstructure of Yak Milk Hard Cheese

SHI Yongqi, LIANG Qi, SONG Xuemei, ZHANG Yan   

  1. (Functional Dairy Product Engineering Laboratory of Gansu Province, College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)
  • Online:2020-10-25 Published:2020-10-23

摘要: 通过对不同脂肪含量的牦牛乳硬质干酪功能特性测定,阐明脂肪含量对牦牛乳硬质干酪质构、流变及微观结构的影响。将脱脂和全脂牦牛乳按0∶1、1∶1、2∶1的体积比调配制成全脂、半脱脂和低脂牦牛乳硬质干酪,测其理化指标,利用质地剖面分析(texture profile analysis,TPA)质构特性,并用SPSS分析脂肪含量对各质构参数的相关性。使用共聚焦激光扫描显微镜观察干酪微观结构,测其在25~80 ℃条件下的流变特性,同时对弹性模量和黏弹特性变化趋势拟合回归方程。脂肪含量影响干酪出品率,全脂干酪>半脱脂干酪>低脂干酪,脂肪含量与水分含量、硬度、弹性和咀嚼性呈显著负相关,相关系数分别为-0.909、-0.851、-0.795和-0.768;与黏聚性呈极显著正相关,相关系数为0.898。全脂、半脱脂和低脂干酪中脂肪颗粒面积分别为1 800.20、1 317.59、792.02 μm2,且脂肪分别占总面积的10.0%、7.3%和4.4%。干酪脂肪含量越低,弹性模量越大;干酪脂肪含量越高,黏弹特性越大;干酪脂肪含量与理化特性、质构、微观结构和流变特性关系密切,实验结果为脂肪含量对牦牛乳硬质干酪影响的机制研究提供参考依据。

关键词: 脂肪含量;牦牛乳硬质干酪;质构;相关性分析;流变学特性

Abstract: The effects of fat content on the texture, rheology and microstructure of yak milk hard cheese were clarified by measuring the functional properties of yak milk cheeses containing different proportions of fat. Full-fat, semi-skimmed and low-fat hard cheeses were made from defatted and whole yak milk mixtures at ratios of 0:1, 1:1 and 2:1, respectively, and their physicochemical indicators were tested and texture characteristics were analyzed using texture profile analysis (TPA). Moreover, the correlation between fat content and each of the measured texture parameter was analyzed. The microstructure of cheese was observed by confocal laser scanning microscopy, and rheological properties were measured in the temperature range of 25–80 ℃. Furthermore, a regression equation between elastic modulus and viscoelasticity and temperature was set up. The cheese yield increased with increasing fat content. There were significantly negative correlations between fat content and water content, hardness, elasticity or chewiness with correlation coefficients of ?0.909, ?0.851, ?0.795 and ?0.768, respectively, while it was significantly positively correlated with cohesiveness with a correlation coefficient of 0.898. The areas of fat granules in full-fat, semi-skimmed and low-fat cheese were 1 800.20, 1 317.59 and 792.02 μm2, respectively, accounting for 10.0%, 7.3% and 4.4% of the total area, respectively. The lower the fat content, the greater the elastic modulus; conversely, the higher the fat content, the greater the viscoelasticity. The fat content was closely related to the physicochemical properties, texture, microstructure and rheological properties of cheese. This experiment provides a basis for the study of the mechanism of the effect of fat content on yak milk cheese.

Key words: fat content; yak milk hard cheese; texture; correlation analysis; rheological properties

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