食品科学 ›› 2020, Vol. 41 ›› Issue (24): 281-286.doi: 10.7506/spkx1002-6630-20190705-074

• 安全检测 • 上一篇    下一篇

侧向免疫层析快速检测牛乳中四环素和青霉素

吴玉晗,陈伟   

  1. (合肥工业大学食品与生物工程学院,安徽 合肥 230009)
  • 出版日期:2020-12-25 发布日期:2020-12-28
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2018YFC1603606)

Rapid Detection of Tetracycline and Penicillin in Milk by Lateral Immunochromatography

WU Yuhan, CHEN Wei   

  1. (School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China)
  • Online:2020-12-25 Published:2020-12-28

摘要: 为快速检测牛乳中残留的四环素和青霉素,应用胶体金免疫层析法,建立可以同时对四环素和青霉素进行检测的双重胶体金试纸条检测方法。结果表明:胶体金侧向免疫层析法用于检测牛乳中的四环素和青霉素,检出限均为2 ng/mL,检测时间10 min,方法特异性较好,与牛乳中常见的其他检测物无交叉反应。该方法简单可靠,为现场快速检测牛乳中的抗生素残留提供一种简单有效的方法。

关键词: 牛乳;四环素;青霉素;多重;快速检测

Abstract: A rapid double colloidal gold immunochromatographic test strip was developed for the simultaneous detection of residual tetracycline and penicillin in milk samples. The results showed that the detection limit for both tetracycline and penicillin was 2 ng/mL, and the detection time was 10 min. This method had good specificity, and exhibited no cross-reaction with other analytes commonly found in milk. The method is simple and reliable, and provides a simple and effective tool for the rapid and on-site detection of antibiotic residues in milk.

Key words: milk; tetracycline; penicillin; multiple; rapid detection

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