食品科学 ›› 2021, Vol. 42 ›› Issue (11): 137-143.doi: 10.7506/spkx1002-6630-20200620-271

• 营养卫生 • 上一篇    下一篇

不同蛋白质摄入量对限能超重大鼠肝脏脂联素信号通路的影响

何智燕,田颖,彭苏文,王倩,陈敏,周环   

  1. (扬州大学食品科学与工程学院,江苏 扬州 225127)
  • 出版日期:2021-06-15 发布日期:2021-06-29
  • 基金资助:
    国家自然科学基金面上项目(81472963)

Effects of Different Protein Intakes on Adiponectin Signaling Pathway in Liver of Overweight Rats Subjected to Limited Energy

HE Zhiyan, TIAN Ying, PENG Suwen, WANG Qian, CHEN Min, ZHOU Huan   

  1. (School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China)
  • Online:2021-06-15 Published:2021-06-29

摘要: 目的:研究不同蛋白质摄入量对低能量摄入状态下的SD超重雄性大鼠肝脏脂联素信号通路的影响。方法:36 只SD雄性大鼠随机取6 只为正常对照组(NC),另外30 只为造模组,给予高脂饲料,9 周后选造模成功的16 只大鼠(体质量为NC组平均体质量的1.1 倍以上)随机均分为模型对照组(MC)、低能量低蛋白组(LP)、低能量正常蛋白组(NP)、低能量高蛋白组(HP),进行能量和蛋白控制,喂养9 周,测定各组大鼠的体质量、体脂肪质量、肝质量、肝脏脂肪质量、血清脂联素(adiponectin,APN)质量浓度和肝脏AMP活化蛋白激酶(AMP-activated protein kinase,AMPK)、固醇调节元件蛋白1c(sterol regulatory element binding protein 1c,SREBP-1c)、乙酰辅酶A羧化酶(acetyl-CoA carboxylase,ACC)、脂肪酸合成酶(fatty acid synthase,FAS)、过氧化物酶体增殖物激活受体α(peroxisome proliferators-activated receptors,PPARα)、肉毒碱棕榈酰转移酶-1(carnitine palmitoyl transterase-1,CPT-1)水平。结果:干预9 周后,与MC组相比,干预组体脂肪质量、肝质量、肝脏脂肪质量、肝脏中SREBP-1c、ACC、FAS水平均显著降低(P<0.05),血清APN水平和肝脏中AMPK、PPARα、CPT-1水平均显著升高(P<0.05),3 个干预组之间体质量、体脂肪质量、肝质量、肝脏中AMPK水平无显著性差异(P>0.05),与LP、NP组相比,HP组肝脏脂肪质量显著降低(P<0.05),血清APN和肝脏SREBP-1c、ACC、FAS、PPARα、CPT-1质量浓度显著升高(P<0.05)。结论:能量控制后,超重雄鼠肝脏APN-AMPK、APN-PPARα-CPT-1信号通路中各因子水平都显著提高(P<0.05),在一定程度上减轻了超重大鼠的体质量、肝质量和肝脏脂肪质量;在能量限制一致的情况下,高蛋白摄入能促进APN-PPARα信号通路的激活,从而减轻了超重大鼠的肝脏脂肪质量,而对体质量、体脂肪质量、肝质量影响不显著(P>0.05)。

关键词: 脂联素信号通路, 蛋白质摄入量, 能量限制

Abstract: Objective: To study the effect of different protein intakes on the adiponectin signaling pathway in the liver of overweight male SD rats with low energy intake. Methods: Six out of 36 rats were randomly selected as normal control group (NC), and the rest were given a high-fat diet. After 9 weeks of feeding, 16 rats with average body mass over 1.1 times heavier than that of the normal diet group were selected and equally randomized into model control (MC), low-energy, low-protein (LP), low-energy, normal-protein (NP), low-energy, high-protein (HP) groups before being subjected to energy and protein controlled feeding for 9 weeks. During the experimental period, body mass, body fat mass, liver mass, liver fat mass, serum adiponectin (APN) concentration, and the levels of liver AMP-activated protein kinase (AMPK), sterol regulatory element binding protein 1c (SREBP-1c), acetyl-CoA carboxylase (ACC), fatty acid synthetase (FAS), peroxisome proliferators-activated receptors α (PPARα) and carnitine palmitoy transferase-1 (CPT-1) in the rats in each group were determined. Results: After 9 weeks of intervention, compared with the MC group, body fat mass, liver mass, liver fat mass, and liver SREBP-1c, ACC and FAS levels in the three intervention groups were significantly reduced (P < 0.05), and serum APN level and liver AMPK, PPARα and CPT-1 levels were significantly increased (P < 0.05). There was no significant difference in body mass, body fat mass, liver mass or liver AMPK level among the intervention groups (P > 0.05). Compared with the LP and NP groups, liver fat mass in the HP group was significantly reduced (P < 0.05), and serum APN and liver SREBP-1c, ACC, FAS, PPAR α and CPT-1 levels were increased significantly (P < 0.05). Conclusion: After energy control, the levels of various factors in the signaling pathways of APN-AMPK and APN-PPARα-CPT-1 in the liver of overweight rats were significantly increased (P < 0.05), thereby reducing body mass, liver mass and liver fat mass to some extent. In the case of consistent energy restriction, high protein intake can promote the activation of APN-PPARα signaling pathway, thus reducing live fat mass in overweight rats, while having no significant influence on the body mass, body fat mass or liver mass (P > 0.05).

Key words: adiponectin signaling pathway, protein intake, energy limitation

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