食品科学 ›› 2021, Vol. 42 ›› Issue (14): 151-158.doi: 10.7506/spkx1002-6630-20200629-373

• 成分分析 • 上一篇    下一篇

“清香”与“栗香”绿茶中非挥发性化学成分的差异分析

王梦琪,朱荫,张悦,施江,林智,吕海鹏   

  1. (1.农业农村部茶树生物学与资源利用重点实验室,中国农业科学院茶叶研究所,浙江 杭州 310008;2.中国农业科学院研究生院,北京 100081)
  • 发布日期:2021-07-27
  • 基金资助:
    国家自然科学基金面上项目(31470694);现代农业产业技术体系建设专项(CARS-19); 中国农业科学院科技创新工程项目(CAAS-ASTIP-2014-TRICAAS)

Analysis of Differential Non-volatile Chemical Compounds between Green Teas with Refreshing Aroma and Chestnut-like Aroma

WANG Mengqi, ZHU Yin, ZHANG Yue, SHI Jiang, LIN Zhi, LÜ Haipeng   

  1. (1. Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China)
  • Published:2021-07-27

摘要: 采用超高效液相色谱-串联质谱(ultra-high performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)技术分析“清香”绿茶和“栗香”绿茶中非挥发性化学成分的差异。结果表明,从“栗香”和“清香”绿茶中检测到543 个化合物,其中含量差异较大的成分有173 个,包括黄酮类75 个、酚酸类22 个、萜类1 个、脂质31 个、氨基酸及其衍生物类9 个、生物碱4 个、核苷酸及其衍生物9 个、有机酸5 个、木脂素和香豆素5 个、鞣质1 个以及其他类11 个等11 类物质;“清香”绿茶中含量高于“栗香”绿茶的化合物共63 个,含量低于“栗香”绿茶的化合物共110 个;α-亚麻酸、棕榈油酸、香兰素、隐绿原酸、山柰酚-3-O-芸香糖苷、L-蛋氨酸及γ-氨基丁酸共7 个化合物对绿茶样品中香气品质成分的生成可能有重要影响;“清香”绿茶中隐绿原酸、L-蛋氨酸含量较高,而“栗香”绿茶中α-亚麻酸、棕榈油酸、香兰素、γ-氨基丁酸及山柰酚-3-O-芸香糖苷等成分的含量比较丰富。

关键词: 清香;栗香;绿茶;非挥发性成分;差异化合物

Abstract: The differential non-volatile compounds between green tea with a refreshing aroma and that with a chestnut-like aroma were identified by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A total of 543 chemical compounds were identified in the two tea samples, 173 of which were found to show significantly different contents between the two samples, including 75 flavonoids, 22 phenolic acids, 1 terpene, 31 lipids, 9 amino acids and their derivatives, 4 alkaloids, 9 nucleotides and their derivatives, 5 organic acids, 5 lignans and coumarins, 1 tannin, and 11 other kinds. Out of the compounds common to both teas, 63 were more abundant and 110 were less abundant in green tea with a refreshing aroma than with a chestnut-like aroma. In addition, a total of 7 compounds, namely α-linolenic acid, palmitoleic acid, vanillin, γ-aminobutyric acid, kaempferol-3-O-rutinoside, cryptochlorogenic acid, and L-methionine were identified as important aroma compounds in green tea. Moreover, cryptochlorogenic acid and L-methionine were found to be prominent in green tea with a refreshing aroma, whilst α-linolenic acid, palmitoleic acid, vanillin, γ-aminobutyric acid, and kaempferol-3-O-rutinoside were dominant in green tea with a chestnut-like aroma.

Key words: refreshing aroma; chestnut-like aroma; green tea; non-volatile compounds; differential compounds

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