食品科学 ›› 2021, Vol. 42 ›› Issue (15): 44-49.doi: 10.7506/spkx1002-6630-20200703-036

• 基础研究 • 上一篇    下一篇

红毛丹果皮中老鹳草素的分离纯化及其热稳定性

李钰景,孙丽平,庄永亮   

  1. (昆明理工大学农业与食品学院,云南 昆明 650500)
  • 出版日期:2021-08-15 发布日期:2021-08-27
  • 基金资助:
    国家自然科学基金地区科学基金项目(31960477)

Purification and Thermal Stability of Geraniin from Rambutan (Nephelium lappaceum) Peels

LI Yujing, SUN Liping, ZHUANG Yongliang   

  1. (Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, China)
  • Online:2021-08-15 Published:2021-08-27

摘要: 老鹳草素是一种天然来源的、具有显著生物活性的可水解鞣花单宁,前期研究发现红毛丹果皮富含老鹳草素。为了提高红毛丹果皮的利用率,本实验研究了中压液相色谱反相C18柱系统和制备型高效液相色谱对红毛丹果皮多酚制取物中老鹳草素的快速分离纯化技术,采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱系统对老鹳草素的热稳定性及其热降解产物进行了定性分析。结果发现真空冷冻干燥的红毛丹果皮中老鹳草素含量高达12.67 g /100 g(以果皮干质量计);其多酚制取物经中压液相色谱反向C18柱系统和制备型高效液相色谱系统分离和洗脱,得到纯度为95.63%的老鹳草素产品,产率为6.00%(以果皮干质量计)。纯化后的老鹳草素不耐高温,60 ℃处理10 h,老鹳草素可降解损失17.13%;100 ℃处理2 h,降解损失率为73.97%。在老鹳草素的热降解体系中鉴定出没食子酸、柯里拉京、六羟基联苯二甲酸、云实酸、Galloyl-bis-HHDP-glucose、鞣花酸、云实素等分子质量更低的酚类物质。研究结果可为老鹳草素的可控降解及生物利用提供探讨。

关键词: 红毛丹果皮;老鹳草素;鞣花单宁;分离纯化;热稳定性

Abstract: Geraniin is a naturally occurring hydrolysable ellagitannin with significant biological activity. Earlier, we have demonstrated that rambutan peel is rich in geraniin. In this study, the rapid separation and purification of geraniin from rambutan peel polyphenols (RPP) extract was carried out by medium-pressure liquid chromatography (MPLC) with a reversed-phase C18 column system and preparative high-performance liquid chromatography (HPLC). The identification of geraniin and its thermal degradation products was conducted by ultra-performance liquid chromatography-quadrupole-orbitirp-mass spectrometry (UPLC-Q-Orbitrap-MS). The results showed that the content of geraniin in freeze-dried rambutan peel was 12.67 g/100 g (on a dry matter basis). Geraniin with a purity of 95.63% and a yield of 6.00% (on the basis of the dry mass of rambutan peel) was obtained. The purified geraniin showed instability to heating. The percentage of degradation loss was 17.13% after treatment at 60 ℃ for 10 hours, and was 73.97% after treatment at 100 ℃ for 2 hours. In the degradation products of geraniin, gallic acid, corilagin, hexahydroxybiphthalic acid, brevifolin carboxylic acid, galloyl-bis-HHDP-glucose, ellagic acid, and brevifolin were detected as phenols with lower molecular mass. The results of the study can provide a discussion on the controlled degradation and bioavailability of geraniin.

Key words: rambutan peel; geraniin; ellagitannin; separation and purification; thermal stability

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