不同产地地参中17 种氨基酸的测定与分析

doi:10.7506/spkx1002-6630-20200108-090

食品科学 ›› 2021, Vol. 42 ›› Issue (2): 255-261.doi: 10.7506/spkx1002-6630-20200108-090

不同产地地参中17 种氨基酸的测定与分析

黄小兰，何旭峰，杨勤，谷文超，周祥德，张华，周浓

（1.重庆三峡学院生物与食品工程学院，三峡库区道地药材绿色种植与深加工重庆市工程实验室，重庆 404100；2.重庆市万州食品药品检验所，重庆 404100；3.重庆三峡医药高等专科学校中医学院，重庆 404100）

出版日期:2021-01-18 发布日期:2021-01-27
基金资助:
重庆市自然科学基金项目（cstc2019jcyj-msxmX0770）；重庆市恒物通农业开发有限公司委托项目

Determination of 17 Amino Acids in the Dried Rhizome of Lycopus lucidus Turcz. var. hirtus Regel from Different Habitats

HUANG Xiaolan, HE Xufeng, YANG Qin, GU Wenchao, ZHOU Xiangde, ZHANG Hua, ZHOU Nong

(1. Green Planting and Deep Processing of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing Engineering Laboratory, College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing 404100, China;2. Chongqing Wanzhou Food and Drug Inspection Institute, Chongqing 404100, China;3. College of Traditional Chinese Medicine, Chongqing Three Gorges Medical College, Chongqing 404100, China)

Online:2021-01-18 Published:2021-01-27

摘要/Abstract

摘要： 以全国6 个产地地参为研究对象，建立一种异硫氰酸苯酯柱前衍生，超高效液相色谱法同时测定地参中17 种氨基酸含量的方法，并对不同产地地参中氨基酸类成分进行分析评价。采用ACQUITY UPLC BEH C18色谱柱（2.1 mm×150 mm，1.7 μm）分离，流动相为0.1 mol/L乙酸钠-80%乙腈溶液梯度洗脱（冰醋酸调pH 6.5），检测波长254 nm，柱温40 ℃，流速0.2 mL/min，可在19 min内实现17 种氨基酸衍生物的分离。结果表明，17 种氨基酸在各自质量浓度范围内线性关系良好（r≥0.999 0），平均回收率为89.69%～106.02%，相对标准偏差为0.44%～2.60%，检出限为0.020～0.110 μg/mL，定量限为0.067～0.368 μg/mL。地参水解溶液中均检出17 种氨基酸，其中7 种必需氨基酸和9 种药用氨基酸，氨基酸种类丰富；必需氨基酸占总氨基酸比例为23.99%～29.36%，药用氨基酸占总氨基酸比例为68.44%～74.16%；不同产地间氨基酸种类无差异，但含量差异较大，各产地均以天冬氨酸和谷氨酸含量为最高，平均含量分别为13.338 mg/g和8.478 mg/g，分别占总氨基酸含量的26.32%和16.73%。聚类分析将6 个产地分为3 类，山东菏泽样品总氨基酸含量最高，达到81.663 mg/g；重庆万州样品总氨基酸含量最低，仅19.463 mg/g。氨基酸成分含量差异可能与地域有关。综上所述，地参氨基酸具有重要的营养价值和药用价值，本研究可为深入研究地参药材的品质评价和资源开发利用提供基础资料。

关键词: 地参；氨基酸；异硫氰酸苯酯；超高效液相色谱；必需氨基酸；药用氨基酸

Abstract: An ultra-high performance liquid chromatography (UPLC) method for the simultaneous determination of 17 amino acids in the dried rhizome of Lycopus lucidus Turcz. var. hirtus Regel collected from six different habitats in China was established using pre-column derivatization with phenyl isothiocyanate (PITC). The chromatographic separated was achieved on an ACQUITY UPLC BEH C18 column (2.1 mm × 150 mm, 1.7 μm) within 19 minutes using a mobile phase composed of 0.1 mol/L sodium acetate solution and 80% acetonitrile solution (adjusted to pH 6.5 with acetic acid). The detection wavelength was set at 254 nm, the column temperature at 40 ℃, and the flow rate of sample loading at 0.2 mL/min. The results showed for all the 17 amino acids, a good linear relationship (r ≥ 0.999 0) was obtained within their respective concentration ranges. The average recoveries were 89.69%–106.02% with relative standard deviations (RSDs) ranging from 0.44% to 2.60%. The limits of detection (LODs) were 0.020–0.110 μg/mL, and the limits of quantitation (LOQs) were 0.067–0.368 μg/mL. The 17 amino acids were all detected in the hydrolysate of L. lucidus Turcz. var. hirtus Regel, including seven essential amino acids and nine medicinal amino acids. The ratios of essential amino acids (EAAs) to total amino acids (TAAs) were between 23.99% and 29.36%, and the ratios of medicinal amino acids (MAAs) to TAAs ranged between 68.44% and 74.16%. There existed a significant difference in the contents but not types of amino acid among rhizome samples from different geographical origins. Aspartic and glutamic acid were the most abundant amino acids in all the samples, and their average contents were 13.338 and 8.478 mg/g, accounting for 26.32% and 16.73% of TAAs, respectively. The habitats were divided into 3 categories by cluster analysis. Among the habitats, the samples collected from Heze, Shandong province and Wanzhou district, Chongqing had the highest and lowest amino acid contents (81.663 and only 19.463 mg/g), respectively. In conclusion, the dried rhizome of L. lucidus Turcz. var. hirtus Regel has high nutritional and medicinal value, and this study can provide basic data for the quality evaluation and utilization of this plant.

Key words: Lycopus lucidus Turcz. var. hirtus Regel; amino acids; phenyl isothiocyanate; ultra-high performance liquid chromatography; essential amino acids; medicinal amino acids

中图分类号:

O657.72

黄小兰，何旭峰，杨勤，谷文超，周祥德，张华，周浓. 不同产地地参中17 种氨基酸的测定与分析[J]. 食品科学, 2021, 42(2): 255-261.

HUANG Xiaolan, HE Xufeng, YANG Qin, GU Wenchao, ZHOU Xiangde, ZHANG Hua, ZHOU Nong. Determination of 17 Amino Acids in the Dried Rhizome of Lycopus lucidus Turcz. var. hirtus Regel from Different Habitats[J]. FOOD SCIENCE, 2021, 42(2): 255-261.

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不同产地地参中17 种氨基酸的测定与分析

Determination of 17 Amino Acids in the Dried Rhizome of Lycopus lucidus Turcz. var. hirtus Regel from Different Habitats

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