茶树精油对扩展青霉线粒体功能的影响

doi:10.7506/spkx1002-6630-20200131-297

食品科学 ›› 2021, Vol. 42 ›› Issue (3): 7-13.doi: 10.7506/spkx1002-6630-20200131-297

茶树精油对扩展青霉线粒体功能的影响

陈雪昱，邹秀容，韦莹莹，许凤，王鸿飞，邵兴锋

（1.宁波大学食品与药学学院，浙江宁波 315800；2.韶关学院英东食品学院，广东韶关 512005）

发布日期:2021-02-25
基金资助:
国家自然科学基金面上项目（31371860）

Effect of Tea Tree Oil on Mitochondrial Function of Penicillium expansum

CHEN Xueyu, ZOU Xiurong, WEI Yingying, XU Feng, WANG Hongfei, SHAO Xingfeng

(1. College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China; 2. Yingdong College of Food Science and Engineering, Shaoguan University, Shaoguan 512005, China)

Published:2021-02-25

摘要/Abstract

摘要： 为了揭示茶树精油（tea tree oil，TTO）对果蔬采后病原真菌扩展青霉（Penicillium expansum）的抑菌作用机制，以TTO处理的P. expansum孢子、菌丝和线粒体为研究对象，测定活性氧（reactive oxygen species，ROS）积累及6 种线粒体功能相关酶类的活力变化，并采用扫描电子显微镜、透射电子显微镜观察TTO处理对线粒体形态和超微结构的影响。结果表明：TTO处理后P. expansum线粒体发生严重的皱缩、干瘪，线粒体内有囊泡结构，线粒体基质流失，严重破坏了P. expansum的线粒体结构。同时TTO处理诱导了P. expansum孢子内ROS的大量积累，导致菌丝细胞内ATP含量下降和ATP酶、柠檬酸合酶、异柠檬酸脱氢酶、α-酮戊二酸脱氢酶、苹果酸脱氢酶和琥珀酸脱氢酶活力下降，破坏了P. expansum的三羧酸（tricarboxylic acid，TCA）循环。结果表明TTO处理会引起P. expansum孢子内ROS的积累，破坏菌丝体内的线粒体形态和超微结构，并导致TCA循环和能量代谢异常。可见，线粒体功能的严重受损是TTO抑制P. expansum的重要机制。

关键词: 茶树精油；扩展青霉；活性氧；线粒体

Abstract: In order to study the antifungal mechanism of tea tree oil (TTO) on Penicillium expansum in postharvest fruits and vegetables, the fungal spores, mycelia and mitochondria treated with TTO were tested for the accumulation of reactive oxygen species (ROS) and changes in six enzyme activities associated with mitochondrial function. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were also used to observe the morphology and ultrastructure of mitochondria. The results showed that TTO treatment caused severe wrinkling and shriveling of mitochondria along with the formation of vesicles within mitochondria and a loss of mitochondrial matrix, thereby seriously damaging the mitochondrial structure. Meanwhile, TTO treatment induced the accumulation of a large amount of ROS in P. expansum spores, thus resulting in a decrease in intracellular adenosine triphosphate (ATP) content and the activities of citrate synthetase (CS), isocitrate dehydrogenase (ICDH), α-ketoglutarate dehydrogenase (α-KGDH), malic dehydrogenase (MDH), and succinate dehydrogenase (SDH) in the mycelial cells and blocking the tricarboxylic acid (TCA) cycle. Therefore, TTO treatment can damage mitochondrial structure and function in P. expansum, cause high accumulation of ROS, and consequently lead to the disruption of the TCA cycle and abnormal energy metabolism. Clearly, causing serious damage to mitochondrial function is the primary mechanism by which TTO inhibits P. expansum.

Key words: tea tree oil (TTO); Penicillium expansum; reactive oxygen species (ROS); mitochondria

中图分类号:

TS255.1

陈雪昱，邹秀容，韦莹莹，许凤，王鸿飞，邵兴锋. 茶树精油对扩展青霉线粒体功能的影响[J]. 食品科学, 2021, 42(3): 7-13.

CHEN Xueyu, ZOU Xiurong, WEI Yingying, XU Feng, WANG Hongfei, SHAO Xingfeng. Effect of Tea Tree Oil on Mitochondrial Function of Penicillium expansum[J]. FOOD SCIENCE, 2021, 42(3): 7-13.

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茶树精油对扩展青霉线粒体功能的影响

Effect of Tea Tree Oil on Mitochondrial Function of Penicillium expansum

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