食品科学 ›› 2021, Vol. 42 ›› Issue (9): 85-90.doi: 10.7506/spkx1002-6630-20201216-198

• 营养卫生 • 上一篇    下一篇

连翘叶连翘苷提取物对高脂饮食诱导大鼠肥胖的预防作用

王静,黄庆辰,高敏玥,朱小盼,陈子彤,王少康,孙桂菊   

  1. (东南大学公共卫生学院,环境医学工程教育部重点实验室,江苏 南京 210009)
  • 出版日期:2021-05-15 发布日期:2021-06-02
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2016YFD0500604);江苏省高等学校大学生创新创业训练计划项目(S202010286105)

Preventive Effect of Forsythin from Forsythia suspensa Leaves on Obesity Induced by High-Fat Diet in Rats

WANG Jing, HUANG Qingchen, GAO Minyue, ZHU Xiaopan, CHEN Zitong, WANG Shaokang, SUN Guiju   

  1. (Key Laboratory of Environmental Medical and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China)
  • Online:2021-05-15 Published:2021-06-02

摘要: 目的:研究连翘叶中连翘苷提取物对高脂饮食诱导大鼠肥胖的预防作用。方法:将48 只SD大鼠分为6 组,即空白对照组,模型对照组,连翘苷提取物(本实验室自制,连翘苷质量分数为13.3%)低、中、高剂量组和标准品组。连翘苷提取物低、中、高剂量组分别给予连翘苷提取物0.2、0.4、1.2 g/(kg mb·d),即连翘苷剂量分别为26.6、53.2、159.6 mg/(kg mb·d),标准品组给予连翘苷标准品(连翘苷质量分数按98%计)54.3 mg/(kg mb·d),与中剂量组连翘苷剂量相同。空白对照组给予基础饲料,其余各组给予高脂饲料,连续灌胃6 周,观察连翘苷提取物干预后,肥胖大鼠摄食量、体质量、肥胖指数(Lee’s指数)、脂肪系数和血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、总胆汁酸(total bile acid,TBA)、瘦素(leptin,LP)、游离脂肪酸(free fat acid,FFA)水平以及脂肪组织病理切片。结果:模型对照组的TC、LDL-C、TBA、LP、FFA水平均显著高于空白对照组(P<0.05);脂肪组织病理结果显示,模型对照组与空白对照组相比,脂肪细胞数量减少,脂肪充盈,细胞膨大,且脂肪细胞当量直径、周长和面积显著大于空白对照组(P<0.05);并且内脏脂肪质量及其系数、Lee’s指数、体质量均显著大于空白对照组(P<0.05),表明高脂饮食诱导大鼠肥胖成功。与模型对照组相比,连翘苷提取物能够降低肥胖大鼠的TC、LDL-C、LP、FFA水平;脂肪组织病理结果较模型对照组有所改善,可见脂肪细胞数量增加,脂肪细胞变小,但低剂量组效果不明显,高剂量组效果最显著。与模型对照组相比,低剂量组脂肪细胞的面积显著减小(P<0.05),中剂量组脂肪细胞的周长和面积显著减小(P<0.05),高剂量组脂肪细胞的当量直径、周长和面积显著减小(P<0.05)。连翘苷提取物还能降低肥胖大鼠的内脏脂肪质量及其系数、Lee’s指数、体质量,且高剂量组效果更显著,中剂量组与标准品组对肥胖大鼠干预效果相当。结论:连翘苷提取物能够预防高脂饮食诱导的大鼠肥胖,连翘叶中的连翘苷是其功能成分。

关键词: 连翘叶;连翘苷;高脂饮食;肥胖大鼠;预防肥胖

Abstract: Objective: To study the preventive effect of forsythin extract from Forsythia suspensa leaves on obesity induced by high-fat diet in rats. Methods: A total of 48 SD rats were divided into six groups of blank control, model control, low-, medium- and high-dose forsythin extract treatment (made in our laboratory, containing 13.3% forsythin) and forsythin standard (98% purity) treatment. The rats in the low-, medium- and high-dose forsythin extract groups were orally administered with forsythin extract at doses of 0.2, 0.4 and 1.2 g/(kg mb·d), containing 26.6, 53.2, and 159.6 mg/(kg mb·d) of forsythin, respectively. Forsythin standard was administered at a dose of 54.3 mg/(kg mb·d), similar to the middle-dose forsythin extract. The rats in the blank control group were provided with a basal diet whereas those in the other groups were given a high-fat diet. The administration lasted for 6 weeks. Then, food intake, body mass, obesity index (Lee’s index), fat index, and the serum levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), total bile acid (TBA), leptin (LP) and free fatty acids (FFA) were measured and pathological sections of adipose tissue were examined. Results: The serum levels of TC, LDL-C, TBA, LP, and FFA in the model control group were significantly higher than those in the blank control group (P < 0.05). Pathological examination showed that compared with the blank control group, the number of fat cells in the model control group was reduced, and the adipocytes were full of fat and enlarged with significantly larger equivalent diameter, perimeter and area than those in the blank control group (P < 0.05), besides, fat mass and its coefficient, Lee’s index and body mass were significantly higher than those in the control group, indicating successful obesity induction in rats. In addition, compared with the model control group, the forsythin extract especially at the high dose lowered serum TC, LDL-C, LP and FFA in obese rats, and improved the pathological status of adipose tissue, reducing the number and size of adipocytes, but was ineffective at the low dose. The area of adipocytes in the low-dose forsythin extract group was reduced in contrast to the model control group (P < 0.05), the perimeter and area of adipocytes in the middle-dose group were both decreased (P < 0.05), and the equivalent diameter, perimeter and area in the high-dose group were all decreased. The forsythin extract especially at the high dose lowered the visceral fat mass and coefficient, Lee’s index, and body mass of obese rats, and the middle-dose forsythin extract was as effective as forsythin standard. Conclusion: The forsythin extract can prevent obesity induced by high-fat diet in rats through the forsythin contained in it.

Key words: Forsythia suspensa leaves; forsythin; high-fat diet; obese rats; obesity prevention

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