食品科学 ›› 2022, Vol. 43 ›› Issue (13): 131-139.doi: 10.7506/spkx1002-6630-20210713-143

• 营养卫生 • 上一篇    

美藤果粕可溶性膳食纤维的抗氧化及免疫活性评价

赵文俊,陆思名,彭东,赵超凡,杜冰,黎攀   

  1. (华南农业大学食品学院,广东 广州 510642)
  • 发布日期:2022-07-27
  • 基金资助:
    广东省自然科学基金项目(2020A1515011268);广东省重点领域研发计划项目(2020B020226008); 云南省杜冰专家工作站项目(202005AF150071)

Antioxidant and Immunological Activities of Sacha Inchi Soluble Dietary Fiber

ZHAO Wenjun, LU Siming, PENG Dong, ZHAO Chaofan, DU Bing, LI Pan   

  1. (College of Food Science, South China Agriculture University, Guangzhou 510642, China)
  • Published:2022-07-27

摘要: 本研究对美藤果粕可溶性膳食纤维(sacha inchi soluble dietary fiber,SISDF)进行了抗氧化和免疫活性评价。利用离子色谱、凝胶色谱和傅里叶变换红外光谱等方法对SISDF结构进行表征。结果表明:SISDF的重均分子质量为401 479 Da,是以吡喃糖为主链的大分子多糖。单糖组成中,半乳糖醛酸相对含量最大(46.49%),其次为鼠李糖(18.95%)、木糖(17.60%)和半乳糖(11.25%)等;当SISDF的质量浓度为2 mg/mL时,1,1-二苯基-2-三硝基苯肼(2,2-diphenyl-1-picrylhydrazyl,DPPH)自由基清除率为68.57%、羟自由基清除率为69.83%、2,2’-联氮-双(3-乙基苯并噻唑啉-6-磺酸)(2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基清除率为70.35%,表现出一定的抗氧化能力;在62.5~1 000 μg/mL质量浓度范围内,SISDF能够极显著增强RAW264.7巨噬细胞的增殖活性和吞噬活性(P<0.01),提升RAW264.7巨噬细胞的一氧化氮、白细胞介素(interleukin,IL)-1β、IL-6、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)因子分泌水平。SISDF具有良好的抗氧化活性和免疫活性,具备开发为天然食品抗氧化剂和消炎保健品的潜力。

关键词: 美藤果粕;膳食纤维;抗氧化;免疫活性

Abstract: Sacha inchi soluble dietary fiber (SISDF) was evaluated for its antioxidant and immunological activity. The structure of SISDF was identified by ion chromatography, gel chromatography and Fourier transform infrared (FTIR) spectroscopy. The results showed that the weight-average molecular mass of SISDF was 401 479 Da, which was found to be a large polysaccharide with pyranose as the main chain. The monosaccharide composition analysis showed that SISDF consisted of galacturonic acid (46.49%), rhamnose (18.95%), xylose (17.60%) and galactose (11.25%). At 2 mg/mL, SISDF scavenged 68.57% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, 69.83% of hydroxyl radical and 70.35% of 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation. At concentrations of 62.5–1 000 μg/mL, SISDF enhanced the proliferative and phagocytic activities of RAW264.7 macrophages significantly (P < 0.01) and increased the secretion levels of NO, interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α factors in RAW264.7 macrophages. Therefore, sacha inchi soluble dietary fiber has good antioxidant and immune activity, and thus has the potential to be developed as a natural food antioxidant and anti-inflammatory nutraceutical.

Key words: sacha inchi; dietary fiber; antioxidant; immune activity

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