食品科学 ›› 2024, Vol. 45 ›› Issue (1): 150-157.doi: 10.7506/spkx1002-6630-20230530-275

• 成分分析 • 上一篇    

基于气相色谱-质谱和超高效液相色谱的火麻油中萜烯类、大麻素类成分分析

张瑞婷,蔡尤西,张浩,孙卉,孙晓东,滕亚君,李晓蕾   

  1. (1.昆明理工大学材料科学与工程学院,云南 昆明 650093;2.昆明理工大学分析测试研究中心,云南 昆明 650093; 3.昆明海关技术中心,云南 昆明 650000)
  • 发布日期:2024-02-05
  • 基金资助:
    云南省科技厅重大科技专项(202102AA310041);毒品分析及禁毒技术公安部重点实验室开放课题(YNPC-B2021003)

Analysis of Terpenes and Cannabinoids in Hemp Oil by Gas Chromatography-Mass Spectrometry and Ultra-high Performance Liquid Chromatography

ZHANG Ruiting, CAI Youxi, ZHANG Hao, SUN Hui, SUN Xiaodong, TENG Yajun, LI Xiaolei   

  1. (1. Faculty of Materials Science and Engineering, Kunming University of Science and Technology, Kunming 650093, China; 2. Analytical and Testing Research Center of Kunming University of Science and Technology, Kunming 650093, China; 3. Technology Centre of Kunming Customs, Kunming 650000, China)
  • Published:2024-02-05

摘要: 基于气相色谱-质谱和超高效液相色谱分析方法,对火麻油中特色的萜烯、脂肪酸和大麻素类化学成分进行分析,并利用数据软件进行主成分分析。结果表明,云南火麻油中含有较为丰富的特征风味性萜烯类成分,如β-石竹烯、β-月桂烯、柠檬烯等;火麻油中脂肪酸含量丰富,尤其亚麻酸和亚油酸接近黄金比例,营养价值高;在不同品种、不同工艺火麻油中均检测到微量、多种大麻素成分,脱壳工艺使火麻油中大麻素成分含量明显降低,冷榨工艺更适合火麻油加工。通过本研究对我国火麻油的食品安全性进行讨论并提出建议。最后对萜烯和大麻素进行主成分分析,找出其中的重要区分物质,对4 种火麻油样本进行完美地区分与鉴定。

关键词: 火麻油;萜烯类成分;大麻素类成分;主成分分析;气相色谱-质谱法;超高效液相色谱

Abstract: Gas chromatography-mass spectrometry (GC-MS) and ultra-high performance liquid chromatography (UPLC) were used to analyzed terpenes, fatty acids and cannabinoids in hemp oil, and the obtained data were analyzed by means of principal component analysis (PCA). The results showed that hemp oil produced in Yunnan contained rich characteristic flavor terpenes including β-caryophyllene, β-myrcene, and limonene. Also, it was rich in fatty acids; particularly the ratio of linolenic acid and linoleic acid was close to the golden ratio, indicating high nutritional value. Trace amounts of multiple cannabinoids were detected in hemp oil irrespective of cultivar or processing method. The content of cannabinoids in hemp oil was significantly reduced by dehulling, and cold pressing was more suitable for hemp oil processing. Moreover, this study discussed the safety of hemp oil for consumption in China and put forward some suggestions. Finally, four hemp oil samples were perfectly distinguished and identified by PCA.

Key words: hemp oil; terpenes; cannabinoids; principal component analysis; gas chromatography-mass spectrometry; ultra-high performance liquid chromatography

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