ε-聚赖氨酸对西番莲果实采后病害与抗病物质代谢的影响

doi:10.7506/spkx1002-6630-20230406-043

食品科学 ›› 2024, Vol. 45 ›› Issue (3): 142-149.doi: 10.7506/spkx1002-6630-20230406-043

ε-聚赖氨酸对西番莲果实采后病害与抗病物质代谢的影响

林育钊，陈蕾伊，陈佳怡，蒋璇靓，郑金水，陈洪彬

（1.泉州师范学院海洋与食品学院，福建泉州 362000；2.泉州市农业科学研究所，福建泉州 362212；3.近海资源生物技术福建省高校重点实验室（泉州师范学院），福建泉州 362000）

出版日期:2024-02-15 发布日期:2024-03-06
基金资助:
福建省自然科学基金面上项目（2023J01902；2021J01976）

Effect of ε-Poly-L-lysine on Postharvest Diseases and Disease-Resistant Substance Metabolism in Passion Fruits

LIN Yuzhao, CHEN Leiyi, CHEN Jiayi, JIANG Xuanjing, ZHENG Jinshui, CHEN Hongbin

(1. College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, China; 2. Quanzhou Institute of Agricultural Sciences, Quanzhou 362212, China; 3. Key Laboratory of Inshore Resources Biotechnology (Quanzhou Normal University), Fujian Province University, Quanzhou 362000, China)

Online:2024-02-15 Published:2024-03-06

摘要/Abstract

摘要： 为研究ε-聚赖氨酸（ε-poly-L-lysine，ε-PL）抑制可可毛色二孢（Lasiodiplodia theobromae）侵染所致黄金西番莲果实采后病害的发生及其与果实采后抗病性的关系，以‘福建百香果3号’黄金西番莲果实为研究对象，以无菌水浸泡处理10 min后接种L. theobromae的西番莲果实为对照组，以100 mg/L ε-PL溶液浸泡处理10 min后接种L. theobromae的西番莲果实为处理组，在（28±1）℃、90%相对湿度下贮藏7 d，每天取样测定果实病斑直径、果皮细胞膜透性、木质素含量和抗病相关酶活性等的变化。结果表明，与接种L. theobromae的西番莲果实对比，ε-PL可延缓果皮细胞膜透性与果实病斑直径的上升。此外，ε-PL还能提高接种L. theobromae西番莲果实的果皮木质素含量，提高果实果皮肉桂酸-4-羟化酶、多酚氧化酶、苯丙氨酸解氨酶、过氧化物酶、β-1,3-葡聚糖酶、4-香豆酰辅酶A连接酶、几丁质酶、肉桂醇脱氢酶等抗病相关酶活性。因此，ε-PL通过减缓西番莲果实果皮细胞膜透性的上升而保持果皮细胞膜的完整性，还能提高西番莲果实果皮抗病相关酶活性及促进木质素累积，进而提升果实采后抗病性，抑制L. theobromae侵染所致采后病害的发生。

关键词: 西番莲果实；ε-聚赖氨酸；可可毛色二孢；采后病害；抗病物质代谢

Abstract: In order to investigate the inhibitory effect of ε-poly-L-lysine (ε-PL) on the occurrence of Lasiodiplodia theobromae-induced diseases and its correlation with disease resistance in postharvest passion fruits, harvested golden passion fruits (cv. Fujian Baixiangguo 3) were immersed in either sterile distilled water (as control group) or 100 mg/L ε-PL solution for 10 min, inoculated with L. theobromae, and stored at (28 ± 1) ℃ and 90% relative humidity for up to 7 days. The changes of fruit lesion diameter, cell membrane permeability, lignin content and disease resistance-related enzyme activities in the pericarp were assayed every day. The results showed that compared to the control group, ε-PL delayed the increase in cell membrane permeability and lesion diameter, and enhanced the content of lignin and the activities of disease resistance-related enzymes including cinnamate-4-hydroxylase (C4H), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), peroxidase (POD), β-1,3-glucanase (GLU), 4-coumarate CoA ligase (4-CL), chitinase (CHI) and cinnamyl alcohol dehydrogenase (CAD), thereby maintaining cell membrane integrity by delaying the increase of cell membrane permeability, enhancing disease resistance, and inhibiting the occurrence of L. theobromae-induced diseases in harvested passion fruits.

Key words: passion fruit; ε-poly-L-lysine; Lasiodiplodia theobromae; postharvest disease; disease-resistant substance metabolism

中图分类号:

TS255.3

林育钊，陈蕾伊，陈佳怡，蒋璇靓，郑金水，陈洪彬. ε-聚赖氨酸对西番莲果实采后病害与抗病物质代谢的影响[J]. 食品科学, 2024, 45(3): 142-149.

LIN Yuzhao, CHEN Leiyi, CHEN Jiayi, JIANG Xuanjing, ZHENG Jinshui, CHEN Hongbin. Effect of ε-Poly-L-lysine on Postharvest Diseases and Disease-Resistant Substance Metabolism in Passion Fruits[J]. FOOD SCIENCE, 2024, 45(3): 142-149.

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ε-聚赖氨酸对西番莲果实采后病害与抗病物质代谢的影响

Effect of ε-Poly-L-lysine on Postharvest Diseases and Disease-Resistant Substance Metabolism in Passion Fruits

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