超声联合高压CO2处理对虾原肌球蛋白结构和致敏性的影响

doi:10.7506/spkx1002-6630-20250110-083

食品科学 ›› 2025, Vol. 46 ›› Issue (16): 295-277.doi: 10.7506/spkx1002-6630-20250110-083

• 食品工程 • 上一篇

超声联合高压CO2处理对虾原肌球蛋白结构和致敏性的影响

杨玉莹，李言初，刘家彤，王泽富，韩宗元，刘书成，魏帅

（1.广东海洋大学食品科技学院，广东省水产品加工与安全重点实验室，广东省海洋生物制品工程实验室，广东省海洋食品工程技术研究中心，广东省水产预制食品加工与品质控制工程技术研究中心，广东?湛江 524088；2.大连工业大学辽宁海洋食品精深加工关键技术省部共建协同创新中心，辽宁?大连 116034）

发布日期:2025-07-22
基金资助:
国家自然科学基金面上项目（32272245）；国家虾蟹产业技术体系项目（CARS-48）

Effect of Combined Ultrasound and High Pressure CO2 Treatment on the Structure and Allergenicity of Shrimp Tropomyosin

YANG Yuying, LI Yanchu, LIU Jiatong, WANG Zefu, HAN Zongyuan, LIU Shucheng, WEI Shuai

(1. Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; 2. Liaoning Marine Food Deep Processing Key Technology Provincial Co-construction Collaborative Innovation Center, Dalian Polytechnic University, Dalian 116034, China)

Published:2025-07-22

摘要/Abstract

摘要： 以凡纳滨对虾原肌球蛋白（tropomyosin，TM）为原料，研究超声联合高压CO2处理对TM结构和致敏性的影响。采用超声功率0～2 000 W、时间0～60 min处理TM，再使用高压CO2（30 MPa、15 min）处理，通过间接酶联免疫吸附测试法和蛋白免疫印迹检测TM致敏性，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定TM分子质量，圆二色光谱、荧光光谱、紫外光谱、游离氨基、总巯基含量表征TM空间结构。结果显示，超声（1 500 W、30 min）联合高压CO2处理后，TM免疫球蛋白（immunoglobulin，Ig）G和IgE的免疫结合活性分别消减了55.3%、48.7%，α-螺旋相对含量降低至42.7%，二级结构由有序变为无序；荧光光谱最大吸收波长发生蓝移，荧光强度减弱，紫外吸收峰发生红移，紫外吸收强度增强；游离氨基含量降低21.82%，总巯基含量降低。综上，超声联合高压CO2处理改变了TM的空间构象，与单独处理相比更好地降低了其致敏性。

关键词: 凡纳滨对虾；原肌球蛋白；超声联合高压CO2；结构；致敏性

Abstract: The combined effect of ultrasound and high pressure carbon dioxide (HPCD) on the structure and allergenicity of the tropomyosin (TM) of Litopenaeus vannamei was studied. After sequential treatment with ultrasonic at powers up to 2 000 W for up to 60 min followed by HPCD at 30 MPa for 15 min, the allergenicity of TM was detected by indirect enzyme-linked immunosorbent assay (iELISA) and Western blot (WB), and the molecular mass was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The spatial structure of TM was characterized by circular dichroism (CD) spectroscopy, fluorescence spectroscopy, ultraviolet (UV) spectroscopy, free amino group and total sulfhydryl group analysis. The results showed that ultrasound at 1 500 W for 30 min combined with HPCD decreased the immunobinding activity of immunoglobulin G (IgG) and IgE in TM by 55.3% and 48.7%, respectively, the α-helix relative content decreased to 42.7%, and the secondary structure was changed from an ordered state to a disordered state. The fluorescence spectra showed a blue shift with weakened fluorescence intensity, and the UV spectra exhibited a red shift with enhanced absorption intensity. The free amino group content was reduced by 21.82%, and the total sulfhydryl group content was also reduced. Therefore, ultrasound combined with HPCD treatment changed the spatial conformation of TM, being more effective in reducing the allergenicity than either single treatment.

Key words: Litopenaeus vannamei; tropomyosin; ultrasound combined with high pressure CO2; structure; allergenicity

中图分类号:

TS254.1

杨玉莹，李言初，刘家彤，王泽富，韩宗元，刘书成，魏帅. 超声联合高压CO2处理对虾原肌球蛋白结构和致敏性的影响[J]. 食品科学, 2025, 46(16): 295-277.

YANG Yuying, LI Yanchu, LIU Jiatong, WANG Zefu, HAN Zongyuan, LIU Shucheng, WEI Shuai. Effect of Combined Ultrasound and High Pressure CO2 Treatment on the Structure and Allergenicity of Shrimp Tropomyosin[J]. FOOD SCIENCE, 2025, 46(16): 295-277.

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超声联合高压CO2处理对虾原肌球蛋白结构和致敏性的影响

Effect of Combined Ultrasound and High Pressure CO2 Treatment on the Structure and Allergenicity of Shrimp Tropomyosin

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