荞麦水溶性多糖的分离纯化及其相对分子量的测定

食品科学 ›› 2009, Vol. 30 ›› Issue (13): 22-24.

荞麦水溶性多糖的分离纯化及其相对分子量的测定

许文涛^1,2，张方方²，罗云波²，王颖²，黄昆仑^1,2,*

1.食品安全分子检测试验室，中国农业大学食品科学与营养工程学院 2.农业部转基因生物食用安全监督检验测试中心

收稿日期:2008-04-09 修回日期:2008-12-03 出版日期:2009-07-01 发布日期:2010-12-29
通讯作者: 许文涛 E-mail:xuwentao1111@sina.com;cauxwt@yahoo.cn
基金资助:
国家“863”计划项目(2006AA10Z440)；农业部“948”项目(2007-Z8)

Isolation and Purification of Water-soluble Polysaccharide from Common Buckwheat (Fagopyum esculentum) and Determination of Its Molecular Weight

XU Wen-tao1,2，ZHANG Fang-fang2，LUO Yun-bo2，WANG Ying2，HUANG Kun-lun1,2,*

1. Laboratory of Food Safety and Molecular Detection, College of Food Science and Nutritional Engineering, China
Agricultural University, Beijing 100083, China；2. Supervision, Inspection and Testing Center of Genetically Modified Food
Safety, Ministry of Agriculture, Beijing 100083, China

Received:2008-04-09 Revised:2008-12-03 Online:2009-07-01 Published:2010-12-29
Contact: XU Wen-tao E-mail:xuwentao1111@sina.com;cauxwt@yahoo.cn

摘要/Abstract

摘要：

通过热水浸提法从荞麦中提取水溶性多糖，并用离子交换柱和凝胶过滤柱对荞麦多糖进行分离纯化、测定其分子量。结果表明，荞麦多糖粗品用Savag 法脱蛋白后，采用DEAE-Sepharose Fast Flow 和Sephacryl S-400HR 柱层析纯化，得到荞麦多糖(FEP)，经过凝胶柱色谱分析表明，FEP 为均一多糖。用苯酚硫酸法测定多糖的含量，用AKTA 快速液相蛋白层析仪的紫外检测器在280nm 波长处检测蛋白质含量。最后根据用不同分子量标准葡聚糖做出的标准曲线，测定出FEP 分子量(Mw)为1720442D。

关键词: 荞麦, 多糖, 分离纯化, 分子量测定

Abstract:

A water-soluble polysaccharide from Fagopyum esculentum (FEP) was obtained by boiling water extraction and ethanol precipitation, followed by consecutive purification on DEAE-Sepharose Fast Flow column and Sephacryl S-400 HR column, and was subjected to freeze-drying. The elution profile of FEP on Sephacryl S-400 HR column presented only a single symmetrical peak, indicating that the FEP was purified to a homogeneous polysaccharide. The polysaccharide content in dried FEP powder was 98.5% determined by phenol-sulfuric acid assay, and no protein components were detected in the powder. The molecular weight of the FEP was calculated as 1720442 D, according to the calibration curve of standard dextrans.

Key words: Fagopyum esculentum, polysaccharide, isolation and purification, determination of molecular weight

中图分类号:

10.7506/spkx1002-6630-200913003

许文涛1,2，张方方2，罗云波2，王颖2，黄昆仑1,2,*. 荞麦水溶性多糖的分离纯化及其相对分子量的测定[J]. 食品科学, 2009, 30(13): 22-24.

XU Wen-tao1,2，ZHANG Fang-fang2，LUO Yun-bo2，WANG Ying2，HUANG Kun-lun1,2,*. Isolation and Purification of Water-soluble Polysaccharide from Common Buckwheat (Fagopyum esculentum) and Determination of Its Molecular Weight[J]. FOOD SCIENCE, 2009, 30(13): 22-24.

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