反相H P L C 同时检测乳酸及乳酸甲酯含量

doi:10.7506/spkx1002-6630-200912034

食品科学 ›› 2009, Vol. 30 ›› Issue (12): 165-167.doi: 10.7506/spkx1002-6630-200912034

反相H P L C 同时检测乳酸及乳酸甲酯含量

姜绍通，刘模，陈小燕，郑志

合肥工业大学生物与食品工程学院

收稿日期:2008-08-29 修回日期:2008-11-22 出版日期:2009-06-15 发布日期:2010-12-29
通讯作者: 姜绍通 E-mail:jiangst@hfut.edu.cn
基金资助:
国家“863”计划项目(2007AA10Z361)

Simultaneous RP-HPLC Determination of Lactic Acid and Methyl Lactate

JIANG Shao-tong，LIU Mo，CHEN Xiao-yan，ZHENG Zhi

(School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China)
Abstract

Received:2008-08-29 Revised:2008-11-22 Online:2009-06-15 Published:2010-12-29
Contact: JIANG Shao-tong， E-mail:jiangst@hfut.edu.cn

摘要/Abstract

摘要：

本实验采用反相高效液相色谱法实现了对乳酸、乳酸甲酯的分离并对其定量分析效果进行研究。HPLC 分析条件为：Purospher STAR C18 反相柱，二极管阵列检测器，以0.005mol/L H2SO4 与甲醇混合溶液(9:1，V/V)为流动相，流速为1.0ml/min，进样量20μl，检测波长210nm。该方法快速、简便、灵敏、重现性好。

关键词: L- 乳酸, 乳酸甲酯, 反相高效液相色谱(RP-HPLC)

Abstract:

A reversed-phase high performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous determination of lactic acid and methyl lactate. The HPLC was equipped with Purospher STAR C18 reversed-phase column and diode array detector. The mobile phase was composed of 0.005 mol/L H2SO4 solution and chromatographically pure methanol (9:1, V/V) with the flow rate of 1.0 ml/min. The injection volume was 20 μl, and the detection wavelength was set as 210 nm. This method is accurate, simple, rapid and reproducible.

Key words: lactic acid, methyl lactate, reverse phase-high performance liquid chromatographic (RP-HPLC)

中图分类号:

TS207.3

姜绍通，刘模，陈小燕，郑志. 反相H P L C 同时检测乳酸及乳酸甲酯含量[J]. 食品科学, 2009, 30(12): 165-167.

JIANG Shao-tong，LIU Mo，CHEN Xiao-yan，ZHENG Zhi. Simultaneous RP-HPLC Determination of Lactic Acid and Methyl Lactate[J]. FOOD SCIENCE, 2009, 30(12): 165-167.

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反相H P L C 同时检测乳酸及乳酸甲酯含量

Simultaneous RP-HPLC Determination of Lactic Acid and Methyl Lactate

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