食品科学 ›› 2009, Vol. 30 ›› Issue (1): 147-150.doi: 10.7506/spkx1002-6630-200901035

• 生物工程 • 上一篇    下一篇

中心组合设计优化基因组改组干酪乳杆菌Lc-F34生产L-乳酸发酵条件研究

王玉华1,2,裴晓林1,李 岩1,王 萍2   

  1. 1.吉林大学 分子酶学工程教育部重点实验室 2.吉林农业大学食品科学与工程学院
  • 收稿日期:2007-11-28 修回日期:2008-07-28 出版日期:2009-01-01 发布日期:2010-12-29
  • 通讯作者: 王玉华 E-mail:yuhua-ww@163.com
  • 基金资助:

    吉林省农业科技发展计划项目(200302-02)

Optimization of L-lactic Acid Fermentation Technology by Genome Shuffled Lactobacillus casei Lc-F34 Using Central Composite Design

WANG Yu-hua1,2,PEI Xiao-lin1,LI Yan1,WANG Ping2   

  1. (1.Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130023,
    China;2.College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)
  • Received:2007-11-28 Revised:2008-07-28 Online:2009-01-01 Published:2010-12-29
  • Contact: WANG Yu-hua1 E-mail:yuhua-ww@163.com

摘要:

随着L- 乳酸在可降解塑料中的应用逐渐增加,乳酸发酵生产受到广泛重视。为了获得较高的生产效率,对于用于工业生产的菌株——基因组改组菌株Lc-F34,优化其发酵工艺是十分必要的。本实验采用中心组合试验设计对干酪乳杆菌基因组改组菌株Lc-F34 发酵工艺进行优化研究,获得该菌株的发酵工艺为:最适pH5.5,度40.2℃。

关键词: 中心组合设计, L- 乳酸, 发酵工艺, 干酪乳杆菌Lc-F34

Abstract:

L-lactic acid is increasingly used as feedstock for potential biodegradable plastics. With the aim to achieve efficient production and high productivity, it is important to optimize the fermentation process for an industrial strain, genome shuffled strain Lc-F34. Temperature and pH play important roles in the lactic acid batch fermentation process. Their effects on L-lactic acid production were studied using central composite design. According to the regression model, the maximum L-lactic acid yield can be obtained at pH 5.5 and 40.2 ℃.

Key words: central composite design, L-lactic acid, fermentation technology, Lactobacillus casei Lc-F34

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