食品科学 ›› 2009, Vol. 30 ›› Issue (12): 254-257.doi: 10.7506/spkx1002-6630-200912058

• 分析检测 • 上一篇    下一篇

量子点标记免疫层析试纸条快速检测莱克多巴胺的研究

张国华,赖卫华,熊勇华,陈 媛   

  1. 南昌大学 食品科学与技术国家重点实验室
  • 收稿日期:2008-07-21 出版日期:2009-06-15 发布日期:2010-12-29
  • 通讯作者: 张国华, E-mail:zgh_206@163.com
  • 基金资助:

    江西省教育厅科学技术研究资助项目(2009)

Application of Quantum Dot Labeling to Development of Immunochromatographic Test Strip for Rapid Detection of Ractopamine

ZHANG Guo-hua,LAI Wei-hua,XIONG Yong-hua,CHEN Yuan   

  1. (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)
  • Received:2008-07-21 Online:2009-06-15 Published:2010-12-29
  • Contact: ZHANG Guo-hua, E-mail:zgh_206@163.com

摘要:

本实验通过莱克多巴胺抗原抗体的制备、量子点的制备、试纸条的组装和测试等步骤研究一种将量子点应用于莱克多巴胺免疫层析试纸条的新方法。结果表明,莱克多巴胺快速检测试纸条的检测限为3ng/ml,检测时间为10min。本法使用方便、经济,适合于莱克多巴胺现场快速检测。

关键词: 莱克多巴胺, 量子点, 免疫层析试纸条

Abstract:

The quantum dot labeling technique was applied to the development of immunochromatographic test strip for rapid detection of ractopamine. Four steps were involved in the procedure of preparation of the strip, including the preparation of antigen and antibody against ractopamine, the production of quantum dots, and the installation and testing of the strip. The detection limit for ractopamine by this strip was 3 ng/ml, and the detection could be finished within 10 min. Apart from being time-saving, the strip is easy to perform and relatively inexpensive. It is therefore ideally suited for on-site testing of ractopamine.

Key words: ractopamine, quantum dots, immunochromatographic test strip

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