食品科学 ›› 2009, Vol. 30 ›› Issue (4): 76-80.doi: 10.7506/spkx1002-6630-200904011

• 工艺技术 • 上一篇    下一篇

鸡胸软骨粉中II型胶原的提取及结构分析

陆雪芹,许时婴*,曹 慧   

  1. 江南大学食品学院
  • 收稿日期:2008-01-30 修回日期:2008-09-01 出版日期:2009-02-15 发布日期:2010-12-29
  • 通讯作者: 陆雪芹 E-mail:lxq_lulutong@yahoo.com.cn

Extraction of Type II Collagen from Chicken Sternal Cartilage Powder and Its Structural Analysis

LU Xue-qin,XU Shi-ying *,CAO Hui   

  1. (School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
  • Received:2008-01-30 Revised:2008-09-01 Online:2009-02-15 Published:2010-12-29
  • Contact: LU Xue-qin E-mail:lxq_lulutong@yahoo.com.cn

摘要:

以鸡胸软骨为原料制备软骨粉,采用盐酸胍溶解去除非胶原成分后,添加胃蛋白酶酶解提取软骨粉II 型胶原。SDS-PAGE 电泳和RP-HPLC 色谱证实了所提取的软骨粉II 型胶原具有较高的纯度。氨基酸组成分析、紫外吸收光谱、FT-IR 谱图、圆二色谱和差示扫描量热分析的测定结果表明软骨粉II 型胶原保持天然构象,结构没有受到破坏。

关键词: 鸡胸软骨, II型胶原, 结构

Abstract:

The material powder was prepared from chick sternal cartilage, hydrochloric carbamidine was used to remove non-collagen components, and then pepsin was added to extract type II collagen. The results of SDS-PAGE electrophoresis and RP-HPLC showed that the extracted type II collagen has high purity. Analysis results of amino acid components, UV absorbance spectrogram, infrared spectrum, circular dichroism (CD) spectrogram and differential scanning calorimeter (DSC) proved that type II collagen from cartilage powder remains its natural structure.

Key words: chicken sternal cartilage, type II collagen, structure

中图分类号: