食品科学 ›› 2011, Vol. 32 ›› Issue (3 ): 56-59.doi: 10.7506/spkx1002-6630-201103014

• 基础研究 • 上一篇    下一篇

桑黄发酵产物的抑菌作用

孟庆龙1,潘景芝2,陈丽1,王琦1,*   

  1. 1.吉林农业大学食药用菌教育部工程研究中心,吉林长春 130118;2.长春市传染病医院,吉林长春 130123
  • 收稿日期:2010-05-21 修回日期:2011-01-06 出版日期:2011-02-15 发布日期:2011-01-13
  • 通讯作者: 孟庆龙 E-mail:mengqinglong1985@sina.com
  • 基金资助:

    长春净月经济开发区资助项目(20060529)

Antibacterial Activity Evaluation of Fermentation Supernatant and Mycelia of Phellinus igniarius

MENG Qing-long1,PAN Jing-zhi2,CHEN Li1,WANG Qi1,*   

  1. 1. Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun
    130118, China;2. Changchun City Hospital for Infectious Disease, Changchun 130123, China
  • Received:2010-05-21 Revised:2011-01-06 Online:2011-02-15 Published:2011-01-13

摘要: 采用滤纸片法和二倍稀释法对桑黄发酵产物中菌丝体不同溶剂提取物和发酵液的抑菌作用及热稳定性进行初步研究。结果表明:桑黄菌丝体甲醇、正丁醇提取物和发酵液对金黄色葡萄球菌、大肠杆菌、肠炎沙门氏菌、鼠伤寒沙门氏菌均有一定的抑制作用,且对金黄色葡萄球菌的抑菌活性表现出较好的热稳定性。与此同时,桑黄菌丝体正丁醇提取物对4 种指示菌的抑菌率均最高,且最低抑菌质量浓度仅为0.1563~0.3125mg/mL,从而说明桑黄菌丝体正丁醇提取物具有较强的抑菌能力。

关键词: 桑黄, 发酵产物, 提取物, 发酵液, 抑菌作用

Abstract: Paper disk and double dilution methods were used to study the inhibition activities and thermal stability of different extracts from mycelia and fermentation broth of Phellinus igniarius. The results showed that the inhibition effects of its methanol, n-butanol extracts of mycelia and fermentation broth on Staphylococcus aureus, Escherichia coli, Salmonellae enteritidis, and Salmonella typhimurium existed, and the extracts were heat stable to Staphylococcus aureus. Meanwhile, the highest rate on inhibition against 4 indicator bacteria was n-butanol extracts from mycelia of P. igniarius, and the minimal inhibitory concentration was 0.1563-0.3125 mg/mL, which showed that its n-butanol extracts had strong antimicrobial activity.

Key words: Phellinys igniarius, fermentation products, extract, fermentation broth, antibacterial activity

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