长双歧杆菌BBMN68 对便秘模型小鼠的通便作用

doi:10.7506/spkx1002-6630-201103045

食品科学 ›› 2011, Vol. 32 ›› Issue (3 ): 195-198.doi: 10.7506/spkx1002-6630-201103045

长双歧杆菌BBMN68 对便秘模型小鼠的通便作用

丁圣¹,蒋菁莉¹,刘松玲²,刘爱萍¹,任发政^2,*

1.蒙牛乳业(北京)有限责任公司，北京 101107；2.中国农业大学食品科学与营养工程学院，北京 100083

收稿日期:2010-06-01 修回日期:2010-12-30 出版日期:2011-02-15 发布日期:2011-01-13
通讯作者: 任发政 E-mail:renfazheng@263.net
基金资助:
“十一五”国家科技支撑计划项目(2006BAD04A06)

Effect of Biofidobactria Strain on Defecating Function in Constipation Mice

DING Sheng1，JIANG Jing-li1，LIU Song-ling2，LIU Ai-ping1，REN Fa-zheng2,*

1. MengNiu Dairy (Beijing) Company, Beijing 101107, China；
2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

Received:2010-06-01 Revised:2010-12-30 Online:2011-02-15 Published:2011-01-13

摘要/Abstract

摘要： 目的：研究长双歧杆菌活菌BBMN68 活菌液对便秘模型小鼠的润肠通便作用。方法：将Balb/c 雄性小鼠随机分为空白组、模型组、阳性对照组和3 个剂量的给药组。给药组分别经口给予低、中、高剂量(1 × 107、1 × 108、1 × 109CFU/mL)的长双歧杆菌BBMB68 活菌液0.1mL/(kg·d)(以体质量计)；空白组和模型组给予0.1mL/(kg·d)的质量分数10% 的脱脂奶溶液；阳性对照组给予1 × 108CFU/mL 动物双歧杆菌菌悬液0.1mL/(kg·d)。用复方地芬诺酯制造小鼠便秘模型，建模后各组分别给药，观察各组小鼠的首次排黑便时间、5h 内排便粒数和质量，测定粪便含水量和小肠墨汁推进率。结果：长双歧杆菌BBMN68 给药7d，可明显改善便秘小鼠首次排黑便时间、增加排黑便粒数和质量；长双歧杆菌BBMN68 给药15d 可显著提高便秘小鼠小肠墨汁推进率。表明长双歧杆菌BBMN68 具有润肠通便功能。

关键词: 双歧杆菌, 通便, 小鼠, 便秘

Abstract: The objective of this study was to evaluate the defecating function of Biofidobacterium animal BBMN68 on constipated mice. Male Balb/c mice were randomly divided into control group, model control group, positive control group and 3 dosages groups. Mice of 3 dosages groups were orally administered with different concentrations of live B. BBMN68 (1 × 107, 1 × 108, 1 × 109 CFU/mL) at 0.1 mL/(kg·d) dose once a day, and mice of control group as well as model control group were administered with 10% skim milk liquid 0.1mL/(kg·d), while the mice of positive control group with 1 × 108 CFU/mL B. animal at 0.1 mL/(kg·d). Mice constipation model was established by using compound diphenoxylate. Each group was respectively treated with corresponding drugs, and then the time of defecation of the first black feces, the character of feces within 5 hours, and the promoting rate of ink were measured. Results: the mice administrated with B. BBMN68 for 7 days had the shorter time of defecation of the first black feces, and the number and weight of mice feces within 5 hours were increased; when the mice were administered with BBMN68 stain for 15 days, the ink propelling rates were increased. Conclusion: B. animal strain BBMN68 strain has the potential defecation function in constipation mice.

Key words: bifidobacteria, defecation, mice, constipation

中图分类号:

R37
TS201.3

丁圣，蒋菁莉，刘松玲，刘爱萍，任发政. 长双歧杆菌BBMN68 对便秘模型小鼠的通便作用[J]. 食品科学, 2011, 32(3 ): 195-198.

DING Sheng，JIANG Jing-li，LIU Song-ling，LIU Ai-ping，REN Fa-zheng. Effect of Biofidobactria Strain on Defecating Function in Constipation Mice[J]. FOOD SCIENCE, 2011, 32(3 ): 195-198.

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长双歧杆菌BBMN68 对便秘模型小鼠的通便作用

Effect of Biofidobactria Strain on Defecating Function in Constipation Mice

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