食品科学 ›› 2009, Vol. 30 ›› Issue (16 ): 189-192.doi: 10.7506/spkx1002-6630-200916039

• 工艺技术 • 上一篇    下一篇

响应面法优化荞麦壳中原花青素的提取工艺

查阳春1,杨义听1,胡晓菡1,李 涛1,2,刘 睿1,*   

  1. 1.华中农业大学食品科技学院 2.湖北省产品质量监督检验研究院
  • 收稿日期:2008-05-18 出版日期:2009-08-15 发布日期:2010-12-29
  • 通讯作者: 刘 睿1,* E-mail:liurui@mail.hzau.edu.cn
  • 基金资助:

    华中农业大学科技创新基金项目(2006-11);华中农业大学SRF 项目(A06076)

Response Surface Optimization of Extraction Technology of Procyanidins from Buckwheat Shells

ZHA Yang-chun1,YANG Yi-ting1,HU Xiao-han1,LI Tao1,2,LIU Rui1,*   

  1. (1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
    2. Hubei Province Supervision and Inspection Institute of Product Quality, Wuhan 430061, China)
  • Received:2008-05-18 Online:2009-08-15 Published:2010-12-29
  • Contact: LIU Rui1,* E-mail:liurui@mail.hzau.edu.cn

摘要:

为充分提取中国苦荞麦壳中的活性多酚,以70% 乙醇- 水为提取剂,通过二次旋转正交试验优化提取工艺,建立了提取得率的二次旋转回归方程,通过响应面分析及岭嵴分析得到了优化组合条件。结果表明,提取温度和时间对提取得率有显著影响(P < 0.05),而料液比对提取得率的影响不显著,较优的提取工艺条件为提取温度73℃、提取时间118.9min、料液比1:16.9,其提取得率0.56%,理论预测值为0.59%,提取得率达到理论预测值的94.9%。

关键词: 荞麦壳, 原花青素, 提取工艺, 旋转正交

Abstract:

Procyanidins in buckwheat shells was extracted with a mixture of 70% ethanol and water. In order to maximize the extraction of procyanidins, a rotatable orthogonal central composite design combined with response surface and ridge analyses was employed. Extraction temperature and time affected the yield of procyanidins significantly (P < 0.05), while the effect of ratio of material to liquid was not significant. The optimum extraction conditions were as follows: ratio of material to liquid 1: 16.9, temperature 73 ℃, and extraction time 118.9 min. Under these conditions, the actual yield of procyanidins was 0.56%, which accounts for 94.9% of the theoretically predicted value of 0.59%.

Key words: buckwheat, procyanidins, extraction technology, rotatable orthogonal central composite design

中图分类号: