食品科学 ›› 2012, Vol. 33 ›› Issue (1): 106-110.doi: 10.7506/spkx1002-6630-201201021

• 生物工程 • 上一篇    下一篇

极大螺旋藻的硒富集化培养研究

杨莹莹,管 斌,孔 青,游  泳,高艳艳,张睿钦,李 肖,左 涛,刘进昌,侯庆云   

  1. 1.中国海洋大学食品科学与工程学院,山东青岛 266003;
    2. 烟台欣和企业食品有限公司研发中心,山东烟台 264006
  • 收稿日期:2011-01-24 修回日期:2011-12-28 出版日期:2012-01-15 发布日期:2012-01-12
  • 通讯作者: 管斌 E-mail:guanbin@ouc.edu.cn
  • 基金资助:

    国家自然科学基金项目(31000823);山东省自然科学基金项目(ZR2010CM050)

Cultivation of Selenium-Enriched Spirulina maxima

YANG Ying-ying,GUAN Bin,KONG Qing,YOU Yong,GAO Yan-yan,ZHANG Rui-qin,LI Xiao,ZUO Tao,LIU Jin-chang,HOU Qing-yun   

  1. 1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;
    2. Research and Development Center, Yantai Shinho Food Co. Ltd., Yantai 264006, China
  • Received:2011-01-24 Revised:2011-12-28 Online:2012-01-15 Published:2012-01-12
  • Contact: GUAN Bin E-mail:guanbin@ouc.edu.cn

摘要:

为得到大量含有机硒的极大螺旋藻(Spirulina maxima),以生物量和藻体内硒富集量作为指标,研究不同硒添加量和硒添加方式对极大螺旋藻生长及富硒效果的影响。结果表明,分8d等量(100μL)多次添加100μg/mL Na2SeO3溶液,是最佳添加量和添加方式,可得到生物量为0.903g/L,单位干藻粉有机硒含量为1413.168μg/g的富硒极大螺旋藻。

关键词: 极大螺旋藻, 硒添加量, 硒添加方式, 生物量, 有机硒含量

Abstract:

 In order to achieve selenium enrichment in Spirulina maxima, the effects of different amounts and strategies for selenium addition on the growth of S. maxima as indicated by biomass and selenium concentration were explored. The addition of 100μL of 100 μg/mL Na2SeO3 at the same time each day for 8 consecutive days after 1 day of growth in Se-free environment was found optimal, resulting in a biomass of 0.903g/L and an organic Se content of 1413.168 μg/g dry S . maxima.

Key words: Spirulina maxima, selenium addition amount, selenium addition mode, biomass, organic selenium content

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