SP825大孔吸附树脂分离提取苦参碱的研究

食品科学 ›› 2007, Vol. 28 ›› Issue (11): 134-138.

SP825大孔吸附树脂分离提取苦参碱的研究

霍清, 林强

北京联合大学生物化学工程学院；北京联合大学生物化学工程学院北京100023；北京100023；

出版日期:2007-11-15 发布日期:2011-11-22

Separation and Purification of Matrine by SP825 Macro-porous Resin

HUO Qing, LIN Qiang

Biochemical Engineering College, Beijing Union University, Beijing 100023, China

Online:2007-11-15 Published:2011-11-22

摘要/Abstract

摘要： 目的:筛选适合分离纯化苦参碱的大孔吸附树脂并确立纯化工艺参数。方法:采用紫外可见分光光度法,静态下考察了7种大孔吸附树脂SP825、D4020、D301R、D152、D201、AB-8和NKA-9的吸附性能。其中SP825吸附量最大,为706.19mg/g。所以选择SP825进行不同浓度的纯化差异考察,并以Frendlich和Langmuir公式拟合进行拟和,拟和效果良好。进一步进行动态研究可由吸附动力曲线得SP825在40min内达平衡。进行洗脱实验由脱附曲线可知乙醇洗脱效果最佳,洗脱条件为:用4BV的50%乙醇进行洗脱,解吸率为81.646%。对苦参碱粗品进行提纯,收率达到27.59%。结论:SP825型大孔吸附树脂综合性能最好,适合于苦参碱的分离纯化。

关键词: 苦参碱, 大孔吸附树脂, 提纯, UV, HPLC

Abstract: Objective: To screen the suitable resin to separate and purify the matrine and to establish the optimum technological parameters of purification. Methods: The adsorption capability of seven different macroresins SP825, D4020, D301R, D152, D201, AB-8, NKA-9 in static state were studied by UV. In these resins, the SP825 resin have the most adsorption capicity, 706.19 mg/g. Therefore SP825 resin was selected to do the different capacities experiment. The different capacities could be described with both of the Frundich and Langmuir isotherms equations. According to dynamics adsorption curve, the balance time was 40 minutes, and the balance time is shorter. 50% ethanol was the best eluantis, and the appropriate desorption conditions were volume 4 BV, and the desorption rate reached 81.646%. Matrine was separated from natural plants with it, and the results also showed that SP825 macroporous adsobent resin could well separate matrine under the conditions listed above, with a total matrinerecovery rate of over 27.59%. Conclusion: The SP825 type macroreticular adsorbent showed better comprehensive adsorption property. It can be used to purify the matrine.

Key words: matrine, macroporous resin, separation, UV, HPLC

霍清, 林强. SP825大孔吸附树脂分离提取苦参碱的研究[J]. 食品科学, 2007, 28(11): 134-138.

HUO Qing, LIN Qiang. Separation and Purification of Matrine by SP825 Macro-porous Resin[J]. FOOD SCIENCE, 2007, 28(11): 134-138.

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