食品科学 ›› 2007, Vol. 28 ›› Issue (12): 275-278.

• 生物工程 • 上一篇    下一篇

香菇双单杂交菌株富铁及其培养条件研究

 叶明, 叶崇军, 陈辉, 王文敏   

  1. 合肥工业大学生物与食品工程学院; 合肥工业大学生物与食品工程学院 安徽合肥230009; 安徽合肥230009;
  • 出版日期:2007-12-15 发布日期:2011-11-22

Study on Optimization of Cultivation Conditions in Iron-enriched Strains of Di-Mon Mating in Lentinula edodes

 YE  Ming, YE  Chong-Jun, CHEN  Hui, WANG  Wen-Min   

  1. College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
  • Online:2007-12-15 Published:2011-11-22

摘要: 对香菇双单杂交菌株NN22、SS22、QS29及单核体菌株S2进行了富铁培养,其生长耐受铁的浓度范围为0.01~0.04mg/ml。选择高富铁率的NN22菌株进行了培养条件优化,结果表明:NN22菌株在PDY培养基中富铁的适宜培养条件为0.02mg/mlFe2+,25℃,pH7.0;在此条件下,1g菌丝(干重)的铁含量达到348μg,多糖含量为5.18mg。结果还表明,当[Fe2+]≤0.02mg/ml时,Fe2+对NN22菌株酯酶同工酶有激活作用。

关键词: 香菇, 富铁, 培养条件, 多糖, 酯酶同工酶

Abstract: Three strains(NN22, SS22, QS29) of di-mon mating and monokaryon strain S2 of Lentinula edodes were iron-enriched in the medium with Fe2+ content ranged from 0.01 to 0.04 mg/ml. The optimization of cultivation conditions of NN22 strain, which is highest yield of iron-enriched one was selected. The results showed that the highest Fe2+ content of NN22 strain was obtained within 20 days at 25℃ and initial pH7.0 in 0.02 mg/ml Fe2+ of PDY. Under the optimized cultivation conditions, Fe2+ content and polysaccharide content of NN22 strain reached 348μg/g and 5.18 mg/g respectively. The results also showed that esterase isoenzyme of NN22 strain was activated when the concentration of iron was less than 0.02 mg/ml.

Key words: Lentinula edodes, iron-enriched, cultivation condition, polysaccharides, esterase isozyme