食品科学 ›› 2004, Vol. 25 ›› Issue (5): 115-119.

• 工艺技术 • 上一篇    下一篇

蛋黄卵磷脂的制备研究

 吴晓英, 林影, 叶倩君, 李晶晶   

  1. 华南理工大学生物工程系
  • 出版日期:2004-05-15 发布日期:2011-10-24

Study on Preparation and Quality Analysis of Egg Yolk Lecithin

 WU  Xiao-Ying, LIN  Ying, YE  Qian-Jun, LI  Jing-Jing   

  1. Department of Bioengineering, South China University of Technology
  • Online:2004-05-15 Published:2011-10-24

摘要: 研究了鸡蛋卵黄卵磷脂的制备工艺和产品质量分析。结果表明,使用95%乙醇分三次提取粗卵磷脂效果最佳,可制得卵磷脂含量约为70%的粗卵磷脂,得率约为10%;进一步使用ZnCl2沉淀制备卵磷脂效果最佳,使用10% ZnCl2纯化粗卵磷脂的回收率约为80%,产品中卵磷脂含量达94.25%;薄层层析结果表明,卵磷脂产品只有两个明显的斑点,分别为PC及PE两种成分。分光光度法测得卵磷脂的紫外最大吸收峰在215nm波长处。

关键词: 卵黄, 卵磷脂, 有机溶剂, 金属盐沉淀剂, 制备

Abstract: The preparation approach and quality analysis egg yolk lecithin were discussed in this paper. The 70% rough lecithinfrom fresh egg yolk was extracted three times with ethanol under different conditions and precipitated by acetone. 10% ZnCl2precipitator was used to purify rough lecithin further. About 80% recovery of refined lecithin was obtained, with the content ofphospholipid over 90%. The maximum ultraviolet absorb peak of product occurred at 215nm. The TLC results showed that thelecithin prepared had two clear spots, PC and PE.

Key words: egg yolk, lecithin, organic solvent, metal salt precipitant, extraction, purification